BMRB: 4602

Solution Structure of Oxidized Microsomal Rabbit Cytochrome b5. Factors Determining the Heterogeneous Binding of the Heme

Authors

Banci, L., Bertini, I., Rosato, A., Scacchieri, S.

Assembly

CYTOCHROME B5

Entity

1. CYTOCHROME B5 (polymer, Thiol state: not present), 94 monomers, 10860.92 Da Detail

DKDVKYYTLE EIKKHNHSKS TWLILHHKVY DLTKFLEEHP GGEEVLREQA GGDATENFED VGHSTDAREL SKTFIIGELH PDDRSKLSKP METL

2. HEM (non-polymer), 616.487 Da

Total weight

11477.407 Da

Max. entity weight

10860.92 Da

Source organism

Oryctolagus cuniculus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 81.3 %, Completeness (bb): 89.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	81.3 % (548 of 674)	82.2 % (476 of 579)	75.8 % (72 of 95)
Backbone	89.0 % (251 of 282)	93.7 % (179 of 191)	79.1 % (72 of 91)
Sidechain	75.8 % (297 of 392)	76.5 % (297 of 388)	0.0 % (0 of 4)
Aromatic	77.1 % (37 of 48)	78.7 % (37 of 47)	0.0 % (0 of 1)
Methyl	82.6 % (38 of 46)	82.6 % (38 of 46)

1. CYTOCHROME B5

DKDVKYYTLE EIKKHNHSKS TWLILHHKVY DLTKFLEEHP GGEEVLREQA GGDATENFED VGHSTDAREL SKTFIIGELH PDDRSKLSKP METL

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Sample

Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	CYTOCHROME B5	[U-15N]		2.0 ~ 3.0 mM
2	phosphate buffer			1 mM

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Protein Blocks Logo

Calculated from 40 models in PDB: 1DO9, Strand ID: A Detail

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Release date

2000-12-05

Citation 1

Solution structure of oxidized microsomal rabbit cytochrome b5. Factors determining the heterogeneous binding of the heme
Banci, L., Bertini, I., Rosato, A., Scacchieri, S.
Eur. J. Biochem. (2000), 267, 755-766, PubMed 10651812 , DOI: , Abstract

Show more 1 citaion

Citation 2

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guentert, P., Mumenthaler, C., Wuethrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Hide non-primary 1 citaion

Related entities 1. CYTOCHROME B5, : 1 : 2 : 235 entities Detail

Interaction partners 1. CYTOCHROME B5, : 4 interactors Detail

Experiments performed 4 experiments Detail