N-terminal zinc-binding HHCC domain of HIV-2 integrase
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 2:ZN1:ZN | 1:HIS12:NE2 |
2 | metal coordination | sing | 2:ZN1:ZN | 1:HIS16:ND1 |
3 | metal coordination | sing | 2:ZN1:ZN | 1:CYS40:SG |
4 | metal coordination | sing | 2:ZN1:ZN | 1:CYS43:SG |
5 | metal coordination | sing | 2:ZN1:ZN | 1:HIS12:NE2 |
6 | metal coordination | sing | 2:ZN1:ZN | 1:HIS16:ND1 |
7 | metal coordination | sing | 2:ZN1:ZN | 1:CYS40:SG |
8 | metal coordination | sing | 2:ZN1:ZN | 1:CYS43:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.9 % (597 of 657) | 98.6 % (342 of 347) | 77.7 % (192 of 247) | 100.0 % (63 of 63) |
Backbone | 85.0 % (277 of 326) | 98.2 % (109 of 111) | 71.0 % (115 of 162) | 100.0 % (53 of 53) |
Sidechain | 97.1 % (372 of 383) | 98.7 % (233 of 236) | 94.2 % (129 of 137) | 100.0 % (10 of 10) |
Aromatic | 81.8 % (36 of 44) | 100.0 % (22 of 22) | 63.6 % (14 of 22) | |
Methyl | 100.0 % (52 of 52) | 100.0 % (26 of 26) | 100.0 % (26 of 26) |
1. Human immunodeficiency virus type 2 integrase
FLEKIEPAQE EHEKYHSNVK ELSHKFGIPN LVARQIVNSC AQCQQKGEAI HGQVNTemperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | H2O | protons | 4.75 ppm | internal | direct | 0.0 |
15C | DDS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13N | DDS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | H2O | protons | 4.75 ppm | internal | direct | 0.0 |
15C | DDS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13N | DDS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | H2O | protons | 4.75 ppm | internal | direct | 0.0 |
15C | DDS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13N | DDS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |
#1 Model Varian Unity Plus (500 MHz)
#2 Model Bruker AMX (600 MHz)
#3 Model Varian Unity Plus (750 MHz)
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
2 | ZINC (II) ION | 1.1 eq | ||
3 | Tris | [U-2H] | 50 mM | |
4 | NaCl | 150 mM | ||
5 | 2-mercaptoethanol | 2 mM | ||
6 | D2O | 99.99 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Human immunodeficiency virus type 2 integrase | [U-15N] | 3.5 mM | |
8 | ZINC (II) ION | 1.1 eq | ||
9 | Tris | [U-2H] | 50 mM | |
10 | NaCl | 150 mM | ||
11 | 2-mercaptoethanol | 2 mM | ||
12 | H2O | 95 % | ||
13 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
15 | ZINC (II) ION | 1.1 eq | ||
16 | Tris | [U-2H] | 50 mM | |
17 | NaCl | 150 mM | ||
18 | 2-mercaptoethanol | 2 mM | ||
19 | D2O | 5 % |
Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | Human immunodeficiency virus type 2 integrase | [U-15N; U-13C] | 3.5 mM | |
21 | ZINC (II) ION | 1.1 eq | ||
22 | Tris | [U-2H] | 50 mM | |
23 | NaCl | 150 mM | ||
24 | 2-mercaptoethanol | 2 mM | ||
25 | H2O | 95 % | ||
26 | D2O | 5 % |