BMRB: 4664

Sequence-Specific Resonance Assignments of Q83, a Lipocalin Highly Expressed in v-myc-Transformed Avian Fibroblasts

Authors

Kontaxis, G., Matt, T., Schuler, W., Krautler, B., Bister, K., Konrat, R.

Assembly

lipocalin Q83

Entity

1. lipocalin Q83 (polymer, Thiol state: all disulfide bound), 157 monomers, 18077.50 Da Detail

MTVPDRSEIA GKWYVVALAS NTEFFLREKD KMKMAMARIS FLGEDELKVS YAVPKPNGCR KWETTFKKTS DDGEVYYSEE AKKKVEVLDT DYKSYAVIYA TRVKDGRTLH MMRLYSRSPE VSPAATAIFR KLAGERNYTD EMVAMLPRQE ECTVDEV

Formula weight

18077.5 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS59:SG	1:CYS152:SG

Source organism

Coturnix coturnix

Exptl. method

NMR

Refine. method

ARIA/CNS automated refinement using also ambiguous NOE distance constraints;

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 59.5 %, Completeness (bb): 90.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	59.5 % (1100 of 1850)	53.1 % (512 of 965)	61.0 % (444 of 728)	91.7 % (144 of 157)
Backbone	90.9 % (845 of 930)	88.9 % (279 of 314)	91.4 % (425 of 465)	93.4 % (141 of 151)
Sidechain	36.8 % (394 of 1071)	35.8 % (233 of 651)	38.2 % (158 of 414)	50.0 % (3 of 6)
Aromatic	1.3 % (2 of 150)	1.3 % (1 of 75)	0.0 % (0 of 73)	50.0 % (1 of 2)
Methyl	26.6 % (42 of 158)	26.6 % (21 of 79)	26.6 % (21 of 79)

1. Q83

MTVPDRSEIA GKWYVVALAS NTEFFLREKD KMKMAMARIS FLGEDELKVS YAVPKPNGCR KWETTFKKTS DDGEVYYSEE AKKKVEVLDT DYKSYAVIYA TRVKDGRTLH MMRLYSRSPE VSPAATAIFR KLAGERNYTD EMVAMLPRQE ECTVDEV

Show sample condition

Sample

Temperature 299 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Q83	[U-13C; U-15N]		3.0 mM
2	potassium phosphate			20 mM
3	potassium chloride			50 mM
4	dithiothreitol			0.5 mM

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LACS Plot; CA

Referencing offset: -0.98 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.98 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.49 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1JZU, Strand ID: A Detail

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Release date

2000-07-07

Citation 1

Sequence-specific resonance assignments of Q83, a lipocalin highly expressed in v-myc-transformed avian fibroblasts
Kontaxis, G., Matt, T., Schuler, W., Krautler, B., Bister, K., Konrat, R.
J. Biomol. NMR (2000), 17, 177-178, PubMed 10921782 , Abstract

Show more 2 citaions

Citation 2

Suppression in transformed avian fibroblasts of a gene (crp) encoding a cysteine-rich protein containing LIM domains
Weiskirchen, R., Bister, K.
Oncogene (1993), 8, 2317-2324, PubMed 8361751 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

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Related entities 1. lipocalin Q83, : 1 : 2 : 1 : 221 entities Detail

Experiments performed 8 experiments Detail