BMRBj - BMREntryMaster

Found 1 Document (1.357 seconds)

BMRB: 4713

1E0M

Structural analysis of WW domains and design of a WW prototype

Authors

Macias, M.J.

Assembly

Prototype WW domain

Entity

1. Prototype WW domain (polymer, Thiol state: not present), 37 monomers, 4352.687 Da Detail

SMGLPPGWDE YKTHNGKTYY YNHNTKTSTW TDPRMSS

Formula weight

4352.687 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 86.7 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	86.7 % (196 of 226)	86.7 % (196 of 226)
Backbone	93.2 % (69 of 74)	93.2 % (69 of 74)
Sidechain	83.6 % (127 of 152)	83.6 % (127 of 152)
Aromatic	90.6 % (29 of 32)	90.6 % (29 of 32)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. prototype

SMGLPPGWDE YKTHNGKTYY YNHNTKTSTW TDPRMSS

Show sample condition

Sample

Temperature 295 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	prototype			2.0 ~ 5.0 mM

Release date

2000-06-14

Citation

Structural analysis of WW domains and design of a WW prototype
Macias, M.J., Gervais, V., Civera, C., Oschkinat, H.
Nat. Struct. Biol. (2000), 7, 375-379, PubMed 10802733 , DOI 10.1038/75144 , Abstract

Related entities 1. Prototype WW domain, : 1 : 486 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	prototype_ww	Warning	210		94.6 (chain: 1, length: 37)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	226	194	85.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	69	93.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	152	125	82.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	8	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	32	29	90.6

Keywords NMR, protein design, signal transduction, WW domain

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4713_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.357 seconds)

BMRB: 4713

Sample

Related entities 1. Prototype WW domain, : 1 : 486 entities Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail