BMRBj - BMREntryMaster

Found 1 Document (5.286 seconds)

BMRB: 4714

2NNT

Structural analysis of WW domains and design of a WW prototype

Authors

Macias, M.J.

Assembly

Formin binding WW domain

Entity

1. Formin binding WW domain (polymer, Thiol state: not present), 40 monomers, 4634.993 Da Detail

GSMGATAVSE WTEYKTADGK TYYYNNRTLE STWEKPQELK

Formula weight

4634.993 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 90.9 %, Completeness (bb): 87.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	90.9 % (220 of 242)	90.9 % (220 of 242)
Backbone	87.8 % (72 of 82)	87.8 % (72 of 82)
Sidechain	92.5 % (148 of 160)	92.5 % (148 of 160)
Aromatic	92.9 % (26 of 28)	92.9 % (26 of 28)
Methyl	100.0 % (15 of 15)	100.0 % (15 of 15)

1. Formin binding WW domain

GSMGATAVSE WTEYKTADGK TYYYNNRTLE STWEKPQELK

Show sample condition

Sample

Temperature 285 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Formin binding WW domain			1.0 ~ 3.0 mM

Release date

2000-06-14

Citation

Structural analysis of WW domains and design of a WW prototype
Macias, M.J., Gervais, V., Civera, C., Oschkinat, H.
Nat. Struct. Biol. (2000), 7, 375-379, PubMed 10802733 , DOI 10.1038/75144 , Abstract

Related entities 1. Formin binding WW domain, : 1 : 21 : 110 entities Detail

Interaction partners 1. Formin binding WW domain, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_1	Warning	253		90.0 (chain: 1, length: 40)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
35	LYS	HZ1	8.843
35	LYS	HZ2	8.843
35	LYS	HZ3	8.843

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	242	220	90.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	82	72	87.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	160	148	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	15	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	28	26	92.9

Keywords WW domain, protein design, signal transduction

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4714_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (5.286 seconds)

BMRB: 4714

Sample

Related entities 1. Formin binding WW domain, : 1 : 21 : 110 entities Detail

Interaction partners 1. Formin binding WW domain, : 7 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail