Determination of the three dimensional structure of the synthetic 113Cd3-beta-C domain of lobster MT-1 by Nuclear Magnetic Resonance
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 2:CD1:CD | 1:CYS11:SG |
2 | metal coordination | sing | 2:CD1:CD | 1:CYS17:SG |
3 | metal coordination | sing | 2:CD1:CD | 1:CYS21:SG |
4 | metal coordination | sing | 2:CD1:CD | 1:CYS28:SG |
5 | metal coordination | sing | 2:CD1:CD | 1:CYS5:SG |
6 | metal coordination | sing | 2:CD1:CD | 1:CYS9:SG |
7 | metal coordination | sing | 2:CD1:CD | 1:CYS27:SG |
8 | metal coordination | sing | 2:CD1:CD | 1:CYS28:SG |
9 | metal coordination | sing | 2:CD1:CD | 1:CYS2:SG |
10 | metal coordination | sing | 2:CD1:CD | 1:CYS5:SG |
11 | metal coordination | sing | 2:CD1:CD | 1:CYS21:SG |
12 | metal coordination | sing | 2:CD1:CD | 1:CYS25:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 96.3 % (158 of 164) | 96.3 % (158 of 164) |
Backbone | 100.0 % (57 of 57) | 100.0 % (57 of 57) |
Sidechain | 94.4 % (101 of 107) | 94.4 % (101 of 107) |
Methyl | 100.0 % (4 of 4) | 100.0 % (4 of 4) |
1. metallothionein
PCEKCTSGCK CPSKDECAKT CSKPCSCCPTTemperature 298 (±1) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | metallothionein | [U-99% 113Cd] | 1.0 ~ 2.0 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 52.4 % (172 of 328) | 52.4 % (172 of 328) |
Backbone | 51.8 % (59 of 114) | 51.8 % (59 of 114) |
Sidechain | 52.8 % (113 of 214) | 52.8 % (113 of 214) |
Methyl | 50.0 % (4 of 8) | 50.0 % (4 of 8) |
1. metallothionein
PCEKCTSGCK CPSKDECAKT CSKPCSCCPTTemperature 298 (±1) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | metallothionein | [U-99% 113Cd] | 1.0 ~ 2.0 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 36.4 % (179 of 492) | 36.4 % (179 of 492) |
Backbone | 35.1 % (60 of 171) | 35.1 % (60 of 171) |
Sidechain | 37.1 % (119 of 321) | 37.1 % (119 of 321) |
Methyl | 33.3 % (4 of 12) | 33.3 % (4 of 12) |
1. metallothionein
PCEKCTSGCK CPSKDECAKT CSKPCSCCPTTemperature 298 (±1) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | metallothionein | [U-99% 113Cd] | 1.0 ~ 2.0 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 27.9 % (183 of 656) | 27.9 % (183 of 656) |
Backbone | 27.2 % (62 of 228) | 27.2 % (62 of 228) |
Sidechain | 28.3 % (121 of 428) | 28.3 % (121 of 428) |
Methyl | 25.0 % (4 of 16) | 25.0 % (4 of 16) |
1. metallothionein
PCEKCTSGCK CPSKDECAKT CSKPCSCCPTTemperature 298 (±1) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | metallothionein | [U-99% 113Cd] | 1.0 ~ 2.0 mM |