BMRBj - BMREntryMaster

Found 1 Document (1.333 seconds)

BMRB: 4774

1QFR

Three-dimensional Structure of the Histidine-containing Phosphocarrier Protein (Hpr) from Enterococcus faecalis in Solution

Authors

Maurer, T., Doker, R., Gorler, A., Hengstenberg, W., Kalbitzer, H.R.

Assembly

PHOSPHOCARRIER PROTEIN HPR

Entity

1. PHOSPHOCARRIER PROTEIN HPR (polymer, Thiol state: not present), 89 monomers, 9448.647 Da Detail

MEKKEFHIVA ETGIHARPAT LLVQTASKFN SDINLEYKGK SVNLKSIMGV MSLGVGQGSD VTITVDGADE AEGMAAIVET LQKEGLAEQ

Formula weight

9448.647 Da

Source organism

Enterococcus faecalis

Exptl. method

NMR

Refine. method

ANGULAR SPACE SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.8 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	96.8 % (489 of 505)	96.8 % (489 of 505)
Backbone	100.0 % (186 of 186)	100.0 % (186 of 186)
Sidechain	95.0 % (303 of 319)	95.0 % (303 of 319)
Aromatic	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (57 of 57)	100.0 % (57 of 57)

1. HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN

MEKKEFHIVA ETGIHARPAT LLVQTASKFN SDINLEYKGK SVNLKSIMGV MSLGVGQGSD VTITVDGADE AEGMAAIVET LQKEGLAEQ

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±3) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN			5.0 mM

Protein Blocks Logo

Calculated from 16 models in PDB: 1QFR, Strand ID: A Detail

Release date

2001-05-02

Citation 1

Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution
Maurer, T., Doker, R., Gorler, A., Hengstenberg, W., Kalbitzer, H.R.
Eur. J. Biochem. (2001), 268, 635-644, PubMed 11168402 , DOI: , Abstract

Show more 4 citaions

Citation 2

Active-centre torsion-angle strain revealed in 1.6 A-resolution structure of histidine-containing phosphocarrier protein
Jia, Z., Vandonselaar, M., Quail, J.W., Delbaere, L.T.
Nature (1993), 361, 94-97, PubMed 8421502 , DOI 10.1038/361094a0 , Abstract

Citation 3

Structural studies of histidine-containing phosphocarrier protein from Enterococcus faecalis
Hahmann, M., Maurer, T., Lorenz, M., Hengstenberg, W., Glaser, S., Kalbitzer, H.R.
Eur. J. Biochem. (1998), 252, 51-58, PubMed 9523711 , DOI: , Abstract

Citation 4

The 1.6 A structure of histidine-containing phosphotransfer protein HPr from Streptococcus faecalis
Jia, Z., Vandonselaar, M., Hengstenberg, W., Quail, J.W., Delbaere, L.T.
J. Mol. Biol. (1994), 236, 1341-1355, PubMed 8126724 , DOI: , Abstract

Citation 5

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Hide non-primary 4 citaions

Related entities 1. PHOSPHOCARRIER PROTEIN HPR, : 1 : 3 : 122 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4774_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4774_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_set_1	Warning	609		100.0 (chain: 1, length: 89)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Assigned chemical shifts

Saveframe: chemical_shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 609
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
38	LYS	HZ1	2.93
38	LYS	HZ2	2.93
38	LYS	HZ3	2.93

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	505	486	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	186	185	99.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	319	301	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	60	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	18	100.0

Keywords HISTIDINE CONTAINING PHOSPHCARRIER PROTEIN, ENTEROCOCCUS FAECALIS, NMR, PROTEIN

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Found 1 Document (1.333 seconds)

BMRB: 4774

Sample

Related entities 1. PHOSPHOCARRIER PROTEIN HPR, : 1 : 3 : 122 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail