NMR Structure of Stem-loop SL2 of the HIV-1 Psi RNA Packaging Signal Reveals a Novel A-U-A Base-triple Platform
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.9 % (577 of 964) | 62.9 % (331 of 526) | 51.5 % (187 of 363) | 78.7 % (59 of 75) |
Backbone | 51.2 % (275 of 537) | 48.5 % (111 of 229) | 44.5 % (113 of 254) | 94.4 % (51 of 54) |
Sidechain | 73.3 % (349 of 476) | 74.1 % (220 of 297) | 76.6 % (121 of 158) | 38.1 % (8 of 21) |
Aromatic | 31.1 % (38 of 122) | 29.5 % (18 of 61) | 38.8 % (19 of 49) | 8.3 % (1 of 12) |
Methyl | 100.0 % (18 of 18) | 100.0 % (9 of 9) | 100.0 % (9 of 9) |
1. HIV-1 SL2 stem loop
GGCGACUGGU GAGUACGCC2. NCp7 protein
MQKGNFRNQR KTVKCFNCGK EGHIAKNCRA PRKKGCWKCG KEGHQMKDCT ERQANTemperature 298 (±2) K, Details natural abundance
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HIV-1 SL2 stem loop | 0.6 ~ 1.2 mM | ||
2 | NCp7 protein | 0.6 ~ 1.2 mM |
Temperature 298 (±2) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | HIV-1 SL2 stem loop | [U-13C] | 0.8 mM | |
4 | NCp7 protein | [U-13C; U-15N] | 0.8 mM |
Temperature 298 (±2) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | HIV-1 SL2 stem loop | 0.8 ~ 1.2 mM | ||
6 | NCp7 protein | [U-15N] | 0.8 ~ 1.2 mM |
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 42.2 % (813 of 1928) | 42.8 % (450 of 1052) | 41.2 % (299 of 726) | 42.7 % (64 of 150) |
Backbone | 41.3 % (444 of 1074) | 42.4 % (194 of 458) | 38.2 % (194 of 508) | 51.9 % (56 of 108) |
Sidechain | 44.1 % (420 of 952) | 43.1 % (256 of 594) | 49.4 % (156 of 316) | 19.0 % (8 of 42) |
Aromatic | 41.0 % (100 of 244) | 40.2 % (49 of 122) | 51.0 % (50 of 98) | 4.2 % (1 of 24) |
Methyl | 50.0 % (18 of 36) | 50.0 % (9 of 18) | 50.0 % (9 of 18) |
1. HIV-1 SL2 stem loop
GGCGACUGGU GAGUACGCC2. NCp7 protein
MQKGNFRNQR KTVKCFNCGK EGHIAKNCRA PRKKGCWKCG KEGHQMKDCT ERQANTemperature 298 (±2) K, Details natural abundance
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HIV-1 SL2 stem loop | 0.6 ~ 1.2 mM | ||
2 | NCp7 protein | 0.6 ~ 1.2 mM |
Temperature 298 (±2) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | HIV-1 SL2 stem loop | [U-13C] | 0.8 mM | |
4 | NCp7 protein | [U-13C; U-15N] | 0.8 mM |
Temperature 298 (±2) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | HIV-1 SL2 stem loop | 0.8 ~ 1.2 mM | ||
6 | NCp7 protein | [U-15N] | 0.8 ~ 1.2 mM |