BMRBj - BMREntryMaster

Found 1 Document (1.375 seconds)

BMRB: 4784

1G2H

1H and 15N chemical shift assignments and interproton 3JHNHA coupling constants of the DNA-binding domain of the tyrosine repressor from Haemophilus influenzae, a transcription factor that belongs to the prokaryotic NtrC superfamily

Authors

Wang, Y.Y., Zhao, S.S., Somerville, R.L., Jardetzky, O.O.

Assembly

Tyrosine repressor

Entity

1. Tyrosine repressor (polymer, Thiol state: not present), 61 monomers, 6877.803 Da Detail

SAVISLDEFE NKTLDEIIGF YEAQVLKLFY AEYPSTRKLA QRLGVSHTAI ANKLKQYGIG K

Formula weight

6877.803 Da

Source organism

Haemophilus influenzae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 87.0 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	87.0 % (388 of 446)	85.3 % (325 of 381)	96.9 % (63 of 65)
Backbone	95.7 % (177 of 185)	94.4 % (118 of 125)	98.3 % (59 of 60)
Sidechain	80.8 % (211 of 261)	80.9 % (207 of 256)	80.0 % (4 of 5)
Aromatic	93.9 % (31 of 33)	93.9 % (31 of 33)
Methyl	100.0 % (39 of 39)	100.0 % (39 of 39)

1. repressor

SAVISLDEFE NKTLDEIIGF YEAQVLKLFY AEYPSTRKLA QRLGVSHTAI ANKLKQYGIG K

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	repressor	[U-15N]		2.0 mM

Protein Blocks Logo

Calculated from 32 models in PDB: 1G2H, Strand ID: A Detail

Coupling constant

34 J values in 1 lists
Temperature 298 (±0.1) K, pH 6.5 (±0.1) Detail

Release date

2001-07-16

Citation

Solution structure of the DNA-binding domain of the TyrR protein of Haemophilus influenzae
Wang, Y.Y., Zhao, S.S., Somerville, R.L., Jardetzky, O.O.
Protein Sci. (2001), 10, 592-598, PubMed 11344327 , DOI 10.1110/ps.45301 , Abstract

Related entities 1. Tyrosine repressor, : 1 : 1 : 12 entities Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4784_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4784_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts	OK	470		98.4 (chain: 1, length: 61)
1	Chemical shift references	chemical_shift_reference_one	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 61, Sequence coverage: 98.4%
- Total number of assignments
  - ¹H chemical shifts: 407
  - ¹⁵N chemical shifts: 63
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	381	329	86.4
¹⁵N chemical shifts	67	63	94.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	125	122	97.6
¹⁵N chemical shifts	60	59	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	256	207	80.9
¹⁵N chemical shifts	7	4	57.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	39	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	31	93.9

Keywords Tyr repressor, NMR, DNA-binding domain, Haemophilus influenzae, Helix-turn-helix

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Found 1 Document (1.375 seconds)

BMRB: 4784

Sample

Related entities 1. Tyrosine repressor, : 1 : 1 : 12 entities Detail

Chemical shift validation 3 contents Detail