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Found 1 Document (6.059 seconds)

BMRB: 4788

1FI6

1H,15N, and 13C NMR resonance assignments for the Eps15 Homology domain Resp1

Authors

Kim, S., Feig, L.A., Baleja, J.D.

Assembly

Reps1 EH domain

Entity

1. Reps1 EH domain (polymer, Thiol state: not reported), 97 monomers, 11035.43 Da Detail

ASVGSWKITD EQRQYYVNQF KTIQPDLNGF IPGSAAKEFF TKSKLPILEL SHIWELSDFD KDGALTLDEF CAAFHLVVAR KNGYDLPEKL PESLMPK

Formula weight

11035.43 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 82.3 %, Completeness (bb): 79.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (967 of 1175)	94.1 % (576 of 612)	64.4 % (298 of 463)	93.0 % (93 of 100)
Backbone	79.3 % (452 of 570)	92.2 % (178 of 193)	66.4 % (190 of 286)	92.3 % (84 of 91)
Sidechain	86.1 % (600 of 697)	95.0 % (398 of 419)	71.7 % (193 of 269)	100.0 % (9 of 9)
Aromatic	49.2 % (62 of 126)	95.2 % (60 of 63)	0.0 % (0 of 61)	100.0 % (2 of 2)
Methyl	96.1 % (98 of 102)	94.1 % (48 of 51)	98.0 % (50 of 51)

1. Eps15 homology domain

ASVGSWKITD EQRQYYVNQF KTIQPDLNGF IPGSAAKEFF TKSKLPILEL SHIWELSDFD KDGALTLDEF CAAFHLVVAR KNGYDLPEKL PESLMPK

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.88 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.88 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Release date

2000-12-17

Citation 1

Letter to the Editor: 1H, 15N, and 13C NMR resonance assignments for the Eps15 homology domain of Reps1
Kim, S., Dubelman, A.M., Goonesekera, S., Feig, L.A., Baleja, J.D.
J. Biomol. NMR (2000), 18, 367-368, PubMed , DOI:

Show more 1 citaion

Citation 2

An Eps homology (EH) domain protein that binds to the Ral-GTPase target, RalBP1
Yamaguchi, A., Urano, T., Goi, T., Feig, L.A.
J. Biol. Chem. (1997), 272, 31230-31234, PubMed 9395447 , DOI: , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 4 entries Detail

  Swiss-Prot: O54916 released on 2003-08-15
    Title REPS1_MOUSE Entity RalBP1-associated Eps domain-containing protein 1
  Swiss-Prot: Q62172 released on 2003-08-15
    Title RBP1_MOUSE Entity RalA-binding protein 1
  Swiss-Prot: Q60631 released on 1997-11-01
    Title GRB2_MOUSE Entity Growth factor receptor-bound protein 2
  Swiss-Prot: Q64010 released on 1997-11-01
    Title CRK_MOUSE Entity Adapter molecule crk

Related entities 1. Reps1 EH domain, : 1 : 5 : 231 entities Detail

Interaction partners 1. Reps1 EH domain, : 8 interactors Detail

More details for 8 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 1H-15N NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

2 1H-15N TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

3 HNCA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

4 HN(CO)CA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

5 HNCBCA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

6 HNCB(CO)CA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

7 HNHB

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

8 1H-15N HSQC

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

9 1H-13C HSQC

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

10 CT 1H-13C HSQC experiments were used for stereospecific assignment

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

11 of methyl protons of Val and Leu with 10% 13C and 99% 15N labeled sample.

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

12 (sample_2)

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Eps15 homology domain	[U-99% 13C; U-99% 15N]		1.3 mM

Sample #2

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Eps15 homology domain	[U-10% 13C; U-99% 15N]		1.0 mM

Sample #3

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Eps15 homology domain	[U-15N]-Leu		0.5 mM

Sample #4

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Eps15 homology domain	[U-15N]-Phe		0.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4788_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4788_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1060		95.9 (chain: 1, length: 97)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 95.9%
- Total number of assignments
  - ¹³C chemical shifts: 286
  - ¹H chemical shifts: 681
  - ¹⁵N chemical shifts: 93
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords calcium binding protein, EH domain, EF hand, Ral

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
41	THR	HG1	5.63
66	THR	HG1	5.65

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	463	286	61.8
¹H chemical shifts	612	583	95.3
¹⁵N chemical shifts	102	93	91.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	194	93	47.9
¹H chemical shifts	193	180	93.3
¹⁵N chemical shifts	91	84	92.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	269	193	71.7
¹H chemical shifts	419	403	96.2
¹⁵N chemical shifts	11	9	81.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	61	0	0.0
¹H chemical shifts	63	60	95.2
¹⁵N chemical shifts	2	2	100.0

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Found 1 Document (6.059 seconds)

BMRB: 4788

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 4 entries Detail

Related entities 1. Reps1 EH domain, : 1 : 5 : 231 entities Detail

Interaction partners 1. Reps1 EH domain, : 8 interactors Detail

Experiments performed 12 experiments Detail

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