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BMRB: 4803

1HKO

1H,13C and 15N chemical shifts of bovine ferrous cytochrome b5

Authors

Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.

Assembly

bovine ferrous cytochrome b5

Entity

1. ferrous cytochrome b5 (polymer, Thiol state: not present), 82 monomers, 9461.265 Da Detail

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. HEM (non-polymer), 616.487 Da

Total weight

10077.752 Da

Max. entity weight

9461.265 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (926 of 961)	97.2 % (484 of 498)	95.0 % (358 of 377)	97.7 % (84 of 86)
Backbone	97.7 % (477 of 488)	97.0 % (163 of 168)	97.9 % (235 of 240)	98.8 % (79 of 80)
Sidechain	95.6 % (525 of 549)	97.3 % (321 of 330)	93.4 % (199 of 213)	83.3 % (5 of 6)
Aromatic	87.2 % (82 of 94)	95.7 % (45 of 47)	78.3 % (36 of 46)	100.0 % (1 of 1)
Methyl	98.8 % (83 of 84)	100.0 % (42 of 42)	97.6 % (41 of 42)

1. ferrous cytochrome b5

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	ferrous cytochrome b5	[U-90% 13C; U-90% 15N]		2.0 ~ 9.0 mM
2	PROTOPORPHYRIN IX CONTAINING FE			2.0 ~ 9.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Release date

2000-11-14

Citation 1

NMR studies of the association of cytochrome b5 with cytochrome c
Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.
Biochemistry (2000), 39, 14025-14039, PubMed 11087350 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. ferrous cytochrome b5, : 1 : 4 : 13 : 12 : 232 entities Detail

Interaction partners 1. ferrous cytochrome b5, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC