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Found 1 Document (2.993 seconds)

BMRB: 4829

1JXS

1H, 15N and 13C resonance assignments for the DNA-binding domain of interleukin enhancer binding factor

Authors

Liu, P., Chen, Y., Li, C., Hsieh, Y., Chen, S., Chen, S., Jeng, W., Chuang, W.

Assembly

Interleukin enhancer binding factor

Entity

1. Interleukin enhancer binding factor (polymer, Thiol state: not present), 98 monomers, 11542.98 Da Detail

DSKPPYSYAQ LIVQAITMAP DKQLTLNGIY THITKNYPYY RTADKGWQNS IRHNLSLNRY FIKVPRSQEE PGKGSFWRID PASESKLIEQ AFRKRRPR

Formula weight

11542.98 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Hybrid distance geometry-dynamical simulated annealing method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 79.1 %, Completeness (bb): 81.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.1 % (964 of 1218)	84.9 % (549 of 647)	67.1 % (314 of 468)	98.1 % (101 of 103)
Backbone	81.5 % (466 of 572)	96.9 % (186 of 192)	66.2 % (192 of 290)	97.8 % (88 of 90)
Sidechain	79.2 % (586 of 740)	79.8 % (363 of 455)	77.2 % (210 of 272)	100.0 % (13 of 13)
Aromatic	72.0 % (85 of 118)	71.2 % (42 of 59)	71.9 % (41 of 57)	100.0 % (2 of 2)
Methyl	83.7 % (72 of 86)	83.7 % (36 of 43)	83.7 % (36 of 43)

1. Interleukin enhancer binding factor

DSKPPYSYAQ LIVQAITMAP DKQLTLNGIY THITKNYPYY RTADKGWQNS IRHNLSLNRY FIKVPRSQEE PGKGSFWRID PASESKLIEQ AFRKRRPR

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JXS, Strand ID: A Detail

Release date

2001-11-13

Citation

Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)
Liu, P., Chen, Y., Li, C., Hsieh, Y., Chen, S., Chen, S., Jeng, W., Chuang, W.
Proteins (2002), 49, 543-553, PubMed 12402362 , DOI 10.1002/prot.10227 , Abstract

Related entities 1. Interleukin enhancer binding factor, : 1 : 3 : 4 : 226 entities Detail

Interaction partners 1. Interleukin enhancer binding factor, : 29 interactors Detail

More details for 29 interactors identified by 12 citations

Experiments performed 13 experiments Detail

1 HNCA

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

2 HN(CO)CA

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

3 HNCACB

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

4 CBCA(CO)NH

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

5 HBHANH

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

6 HBHA(CO)NH

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

7 HCCH-TOCSY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

8 HCCH-COSY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

9 1H-15N-TOCSY-HSQC

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

10 1H-15N-NOESY-HSQC

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

11 2D NOESY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

12 2D TOCSY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

13 2D COSY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interleukin enhancer binding factor	[U-13C; U-15N]		2.5 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Interleukin enhancer binding factor	[U-15N]		2.5 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Interleukin enhancer binding factor			2.5 mM

nullKeywords DNA-binding domain, interleukin enhancer binding factor, resonance assignment

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Found 1 Document (2.993 seconds)

BMRB: 4829

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Interleukin enhancer binding factor, : 1 : 3 : 4 : 226 entities Detail

Interaction partners 1. Interleukin enhancer binding factor, : 29 interactors Detail

Experiments performed 13 experiments Detail

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