BMRB: 4830

The Hairpin Structure of the (6)F1(1)F2(2)F2 Fragment from Human Fibronectin Enhances Gelatin Binding

Authors

Pickford, A.R., Smith, S.P., Staunton, D., Boyd, J., Campbell, I.D.

Assembly

fibronectin fragment 6f11f22f2

Entity

1. fibronectin fragment 6f11f22f2 (polymer, Thiol state: all disulfide bound), 160 monomers, 17856.48 Da Detail

YGHCVTDSGV VYSVGMQWLK TQGNKQMLCT CLGNGVSCQE TAVTQTYGGN SNGEPCVLPF TYNGRTFYSC TTEGRQDGHL WCSTTSNYEQ DQKYSFCTDH TVLVQTRGGN SNGALCHFPF LYNNHNYTDC TSEGRRDNMK WCGTTQNYDA DQKFGFCPMA

Formula weight

17856.48 Da

Entity Connection

disulfide 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS31:SG
2	disulfide	sing	1:CYS29:SG	1:CYS38:SG
3	disulfide	sing	1:CYS56:SG	1:CYS82:SG
4	disulfide	sing	1:CYS70:SG	1:CYS97:SG
5	disulfide	sing	1:CYS116:SG	1:CYS142:SG
6	disulfide	sing	1:CYS130:SG	1:CYS157:SG

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.8 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	93.8 % (1028 of 1096)	94.5 % (863 of 913)	90.2 % (165 of 183)
Backbone	98.6 % (483 of 490)	98.2 % (328 of 334)	99.4 % (155 of 156)
Sidechain	89.9 % (545 of 606)	92.4 % (535 of 579)	37.0 % (10 of 27)
Aromatic	100.0 % (106 of 106)	100.0 % (103 of 103)	100.0 % (3 of 3)
Methyl	100.0 % (57 of 57)	100.0 % (57 of 57)

1. 6f11f22f2

YGHCVTDSGV VYSVGMQWLK TQGNKQMLCT CLGNGVSCQE TAVTQTYGGN SNGEPCVLPF TYNGRTFYSC TTEGRQDGHL WCSTTSNYEQ DQKYSFCTDH TVLVQTRGGN SNGALCHFPF LYNNHNYTDC TSEGRRDNMK WCGTTQNYDA DQKFGFCPMA

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	6f11f22f2	[U-98% 15N]		2.0 mM

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Release date

2001-07-31

Citation

The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding
Pickford, A.R., Smith, S.P., Staunton, D., Boyd, J., Campbell, I.D.
EMBO J. (2001), 20, 1519-1529, PubMed 11285216 , DOI 10.1093/emboj/20.7.1519 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. fibronectin fragment 6f11f22f2, : 1 : 4 : 1 : 1 : 14 entities Detail

Interaction partners 1. fibronectin fragment 6f11f22f2, : 91 interactors Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail