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BMRB: 4833

1G6E

1H, 13C, and 15N assignments for the Antifungal protein from Streptomyces tendae Tu901

Authors

Campos-Olivas, R., Horr, I., Bormann, C., Jung, G., Gronenborn, A.

Assembly

Antifungal protein from Streptomyces tendae Tu901

Entity

1. Antifungal protein from Streptomyces tendae Tu901 (polymer, Thiol state: all disulfide bound), 87 monomers, 9991.086 Da Detail

MINRTDCNEN SYLEIHNNEG RDTLCFANAG TMPVAIYGVN WVESGNNVVT LQFQRNLSDP RLETITLQKW GSWNPGHIHE ILSIRIY

Formula weight

9991.086 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS25:SG

Source organism

Streptomyces tendae

Exptl. method

NMR

Refine. method

Torsion angle dynamics with DYANA.

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.4 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.4 % (972 of 1019)	95.3 % (502 of 527)	95.1 % (372 of 391)	97.0 % (98 of 101)
Backbone	97.9 % (505 of 516)	98.3 % (174 of 177)	98.0 % (250 of 255)	96.4 % (81 of 84)
Sidechain	93.8 % (548 of 584)	93.7 % (328 of 350)	93.5 % (203 of 217)	100.0 % (17 of 17)
Aromatic	76.1 % (70 of 92)	80.4 % (37 of 46)	69.8 % (30 of 43)	100.0 % (3 of 3)
Methyl	100.0 % (98 of 98)	100.0 % (49 of 49)	100.0 % (49 of 49)

1. Antifungal protein from Streptomyces tendae Tu901

MINRTDCNEN SYLEIHNNEG RDTLCFANAG TMPVAIYGVN WVESGNNVVT LQFQRNLSDP RLETITLQKW GSWNPGHIHE ILSIRIY

Show sample condition

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.821 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.821 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.821 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	proton	4.821 ppm	internal	direct	0.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.67 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1G6E, Strand ID: A Detail

Coupling constant

68 J values in 1 lists
Temperature 293 (±0.1) K, pH 8.0 (±0.1) Detail

Release date

2001-07-10

Citation 1

Solution structure, backbone dynamics and chitin binding of the anti-fungal protein from Streptomyces tendae TU901
Campos-Olivas, R., Horr, I., Bormann, C., Jung, G., Gronenborn, A.M.
J. Mol. Biol. (2001), 308, 765-782, PubMed 11350173 , DOI 10.1006/jmbi.2001.4622 , Abstract

Show more 1 citaion

Citation 2

Characterization of a novel, antifungal, chitin-binding protein from Streptomyces tendae Tü901 that interferes with growth polarity
Bormann, C., Baier, D., Horr, I., Raps, C., Berger, J., Jung, G., Schwarz, H.
J. Bacteriol. (1999), 181, 7421-7429, PubMed 10601197 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Antifungal protein from Streptomyces tendae Tu901, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

2 2D 1H-15N HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

3 2D 1H-13C HSQC/HMQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

4 3D HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

5 3D HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

6 3D CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

7 3D HNHA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

8 3D HNHB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

9 3D 15N-edited NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

10 4D 13C,13C NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Bruker DRX (800 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
2	phosphate		20 mM
3	NaCl		0.1 M
4	EDTA		20 uM
5	NaN3		0.01 %
6	D2O		8.3 %
7	H2O		91.7 %

Sample #2

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
9	phosphate		20 mM
10	NaCl		0.1 M
11	EDTA		20 uM
12	NaN3		0.01 %
13	D2O		8.3 %
14	H2O		91.7 %

Sample #3

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-10% 13C]	0.5 ~ 0.8 mM
16	phosphate		20 mM
17	NaCl		0.1 M
18	EDTA		20 uM
19	NaN3		0.01 %
20	D2O		100 %

Sample #4

Temperature 293 (±0.1) K, pH 8.0 (±0.1)

#	Name	Isotope labeling	Concentration
21	Antifungal protein from Streptomyces tendae Tu901	[U-99% 15N; U-13C]	0.5 ~ 0.8 mM
22	phosphate		20 mM
23	NaCl		0.1 M
24	EDTA		20 uM
25	NaN3		0.01 %
26	D2O		100 %

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Found 1 Document (1.813 seconds)

BMRB: 4833

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Antifungal protein from Streptomyces tendae Tu901, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

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