Solution Structure of Methylophilus methylotrophus Cytochrome C'': Insights into the Structural Basis of Heme-ligand Detachment
DVTNAEKLVY KYTNIAHSAN PMYEAPSITD GKIFFNRKFK TPSGKEAACA SCHTNNPANV GKNIVTGKEI PPLAPRVNTK RFTDIDKVED EFTKHCNDIL GADCSPSEKA NFIAYLLTET KPTK
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 2:HEC_ox1:CAB | 1:CYS49:SG |
2 | metal coordination | sing | 2:HEC_ox1:CAC | 1:CYS52:SG |
3 | metal coordination | sing | 2:HEC_ox1:FE | 1:HIS95:NE2 |
4 | metal coordination | sing | 2:HEC_ox1:FE | 1:HIS53:NE2 |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 97.4 % (724 of 743) | 97.4 % (724 of 743) |
Backbone | 99.6 % (243 of 244) | 99.6 % (243 of 244) |
Sidechain | 96.4 % (481 of 499) | 96.4 % (481 of 499) |
Aromatic | 92.3 % (48 of 52) | 92.3 % (48 of 52) |
Methyl | 100.0 % (62 of 62) | 100.0 % (62 of 62) |
1. CYTOCHROME C''
DVTNAEKLVY KYTNIAHSAN PMYEAPSITD GKIFFNRKFK TPSGKEAACA SCHTNNPANV GKNIVTGKEI PPLAPRVNTK RFTDIDKVED EFTKHCNDIL GADCSPSEKA NFIAYLLTET KPTKTemperature 298 (±1) K, pH 5.6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CYTOCHROME C'' | 3.5 mM | ||
2 | ampicillin | 70 uM | ||
3 | kanamicine | 50 uM | ||
4 | chloramphenicol | 50 uM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 53.2 % (790 of 1486) | 53.2 % (790 of 1486) |
Backbone | 56.1 % (274 of 488) | 56.1 % (274 of 488) |
Sidechain | 51.7 % (516 of 998) | 51.7 % (516 of 998) |
Aromatic | 50.0 % (52 of 104) | 50.0 % (52 of 104) |
Methyl | 53.2 % (66 of 124) | 53.2 % (66 of 124) |
1. CYTOCHROME C''
DVTNAEKLVY KYTNIAHSAN PMYEAPSITD GKIFFNRKFK TPSGKEAACA SCHTNNPANV GKNIVTGKEI PPLAPRVNTK RFTDIDKVED EFTKHCNDIL GADCSPSEKA NFIAYLLTET KPTKTemperature 298 (±1) K, pH 5.6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CYTOCHROME C'' | 3.5 mM | ||
2 | ampicillin | 70 uM | ||
3 | kanamicine | 50 uM | ||
4 | chloramphenicol | 50 uM |