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NMR solution structure of alpha-conotoxin Im1 point mutation variant R7L
Authors
Rogers, J.P., Luginbuhl, P., Pemberton, K., Harty, P., Wemmer, D.E., Stevens, R.C.
Assembly
alpha-conotoxin Im1(R7L)
Entity
1. alpha-conotoxin Im1(R7L) (polymer, Thiol state: all disulfide bound), 13 monomers, 1311.557 Da Detail

GCCSDPLCAW RCX


Formula weight
1311.557 Da
Entity Connection
disulfide 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS2:SG1:CYS8:SG
2disulfidesing1:CYS3:SG1:CYS12:SG

Source organism
Conus imperialis
Exptl. method
NMR
Refine. method
TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMISATION
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.3 %, Completeness: 98.4 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

Total1H
All98.4 % (62 of 63)98.4 % (62 of 63)
Backbone95.8 % (23 of 24)95.8 % (23 of 24)
Sidechain100.0 % (39 of 39)100.0 % (39 of 39)
Aromatic100.0 % (6 of 6)100.0 % (6 of 6)
Methyl100.0 % (3 of 3)100.0 % (3 of 3)

1. alpha-conotoxin Im1(R7L)

GCCSDPLCAW RCX

Sample

Temperature 298 (±1) K, pH 3.7 (±0.2)


#NameIsotope labelingTypeConcentration
1alpha-conotoxin Im1(R7L)2.0 ~ 3.0 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1E75, Strand ID: A Detail


Release date
2008-07-15
Citation
Structure-activity Relationships in a Peptidic Alpha7 Nicotinic Acetylcholine Receptor Antagonist
Rogers, J.P., Luginbuhl, P., Pemberton, K., Harty, P., Wemmer, D.E., Stevens, R.C.
J. Mol. Biol. (2000), 304, 911-926
Related entities 1. alpha-conotoxin Im1(R7L), : 1 : 7 : 15 entities Detail
Interaction partners 1. alpha-conotoxin Im1(R7L), : 1 interactors Detail
Experiments performed 3 experiments Detail
nullKeywords alpha-conotoxin Im1, competitive antagonist, neuronal nicotinic acetylcholine receptor, NMR solution structure, peptide toxin