Conformational Changes in the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase upon Shikimate-3-phosphate Binding
MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.7 % (2375 of 2679) | 89.3 % (1230 of 1377) | 87.6 % (923 of 1054) | 89.5 % (222 of 248) |
Backbone | 93.1 % (1319 of 1416) | 94.5 % (466 of 493) | 91.5 % (636 of 695) | 95.2 % (217 of 228) |
Sidechain | 85.3 % (1261 of 1478) | 86.4 % (764 of 884) | 85.7 % (492 of 574) | 25.0 % (5 of 20) |
Aromatic | 19.0 % (24 of 126) | 27.0 % (17 of 63) | 11.1 % (7 of 63) | |
Methyl | 99.7 % (329 of 330) | 100.0 % (165 of 165) | 99.4 % (164 of 165) |
1. EPSP synthase
MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVETemperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EPSP synthase | [U-15N; U-13C; U70%-2H] | 1.0 mM | |
2 | shikimate-3-phosphate | 1.5 mM | ||
3 | Tris buffer | 50 mM | ||
4 | DTT | 1 mM | ||
5 | NaN3 | 3 mM | ||
6 | pepstatin | 0.0 ~ 0.0 uM | ||
7 | leupeptin | 0.0 ~ 0.0 uM |