BMRBj - BMREntryMaster

Found 1 Document (1.162 seconds)

BMRB: 4870

7SZK

Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit

Authors

Poznanski, J., Zhukow, I., Bolewska, K., Wierzchowski, K.L.

Assembly

region 4 of sigma70 of E. coli RNA polymerase holoenzyme

Entity

1. region 4 of sigma70 of E. coli RNA polymerase holoenzyme (polymer, Thiol state: not present), 106 monomers, 12032.43 Da Detail

GSSHHHHHHS SGLVPRGSHM LELPLDSATT ESLRAATHDV LAGLTAREAK VLRMRFGIDM NTDYTLEEVG KQFDVTRERI RQIEAKALRK LRHPSRSEVL RSFLDD

Formula weight

12032.43 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants, heteronucl_T1_relaxation, heteronucl_T2_relaxation, heteronucl_NOEs

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 87.3 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.3 % (1055 of 1209)	86.2 % (542 of 629)	86.5 % (410 of 474)	97.2 % (103 of 106)
Backbone	97.1 % (612 of 630)	96.3 % (207 of 215)	97.8 % (305 of 312)	97.1 % (100 of 103)
Sidechain	79.4 % (539 of 679)	80.9 % (335 of 414)	76.7 % (201 of 262)	100.0 % (3 of 3)
Aromatic	5.4 % (4 of 74)	5.4 % (2 of 37)	5.4 % (2 of 37)
Methyl	84.7 % (100 of 118)	93.2 % (55 of 59)	76.3 % (45 of 59)

1. region 4 of sigma70 of E. coli RNA polymerase

GSSHHHHHHS SGLVPRGSHM LELPLDSATT ESLRAATHDV LAGLTAREAK VLRMRFGIDM NTDYTLEEVG KQFDVTRERI RQIEAKALRK LRHPSRSEVL RSFLDD

Show sample condition

Sample

Temperature 298 (±1) K, pH 2.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	region 4 of sigma70 of E. coli RNA polymerase	[U-13C; U-15N]		3 mM

Chem. Shift Complete2

Sequence coverage: 22.6 %, Completeness: 51.7 %, Completeness (bb): 57.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.7 % (1249 of 2418)	50.6 % (636 of 1258)	51.6 % (489 of 948)	58.5 % (124 of 212)
Backbone	57.7 % (727 of 1260)	57.0 % (245 of 430)	57.9 % (361 of 624)	58.7 % (121 of 206)
Sidechain	47.0 % (638 of 1358)	47.2 % (391 of 828)	46.6 % (244 of 524)	50.0 % (3 of 6)
Aromatic	6.8 % (10 of 148)	8.1 % (6 of 74)	5.4 % (4 of 74)
Methyl	51.7 % (122 of 236)	55.9 % (66 of 118)	47.5 % (56 of 118)

1. region 4 of sigma70 of E. coli RNA polymerase

GSSHHHHHHS SGLVPRGSHM LELPLDSATT ESLRAATHDV LAGLTAREAK VLRMRFGIDM NTDYTLEEVG KQFDVTRERI RQIEAKALRK LRHPSRSEVL RSFLDD

Show sample condition

Sample

Temperature 298 (±1) K, pH 2.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	region 4 of sigma70 of E. coli RNA polymerase	[U-13C; U-15N]		3 mM

Heteronucl. T₁

94 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Temperature 298 (±1) K, pH 2.8 (±0.2) Detail

Heteronucl. T₂

94 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Temperature 298 (±1) K, pH 2.8 (±0.2) Detail

Heteronucl. NOE

95 NOE values in 1 lists
Value type na, Field strength (¹H) 500 MHz, Temperature 298 (±1) K, pH 2.8 (±0.2) Detail

Heteronucl. T₁/T₂

94 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Temperature 298 (±1) K, pH 2.8 (±0.2) Detail

Coupling constant

66 J values in 1 lists
Temperature 298 (±1) K, pH 2.8 (±0.2) Detail

Release date

2001-06-28

Citation 1

Letter to the Editor: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit
Poznanski, J., Zhukov, I., Bolewska, K., Wierzchowski, K.L.
J. Biomol. NMR (2001), 20, 181-182, PubMed

Show more 3 citaions

Citation 2

The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12
Nucleic Acids Res. (1981), 9, 2889-2903, PubMed 6269063 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 4

1H, 13C and 15N chemical shift referencing in biomolecular NMR
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , Abstract

Hide non-primary 3 citaions

Related entities 1. region 4 of sigma70 of E. coli RNA polymerase holoenzyme, : 1 : 94 : 37 entities Detail

Interaction partners 1. region 4 of sigma70 of E. coli RNA polymerase holoenzyme, : 32 interactors Detail

More details for 32 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC