BMRB: 4880

1H, 15N and 13C resonance assignments of the N-terminal region of calponin

Authors

Bramham, J., Smith, B.O., Uhrin, D., Barlow, P.N., Winder, S.J.

Assembly

calponin CH domain

Entity

1. calponin CH domain (polymer, Thiol state: all free), 108 monomers, 12249.81 Da Detail

MPQTERQLRV WIEGATGRRI GDNFMDGLKD GVILCELINK LQPGSVQKVN DPVQNWHKLE NIGNFLRAIK HYGVKPHDIF EANDLFENTN HTQVQSTLIA LASQAKTK

Formula weight

12249.81 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.1 % (1254 of 1291)	96.6 % (653 of 676)	98.2 % (483 of 492)	95.9 % (118 of 123)
Backbone	96.9 % (620 of 640)	95.9 % (211 of 220)	98.1 % (310 of 316)	95.2 % (99 of 104)
Sidechain	97.7 % (734 of 751)	96.9 % (442 of 456)	98.9 % (273 of 276)	100.0 % (19 of 19)
Aromatic	93.2 % (82 of 88)	93.2 % (41 of 44)	92.9 % (39 of 42)	100.0 % (2 of 2)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. calponin CH domain

MPQTERQLRV WIEGATGRRI GDNFMDGLKD GVILCELINK LQPGSVQKVN DPVQNWHKLE NIGNFLRAIK HYGVKPHDIF EANDLFENTN HTQVQSTLIA LASQAKTK

Show sample condition

Sample

Temperature 291 K

#	Name	Isotope labeling	Type	Concentration
1	calponin CH domain	[U-99% 15N; U-98% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.776 ppm	internal	direct	0.0
13C	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.776 ppm	internal	direct	0.0
13C	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.776 ppm	internal	direct	0.0
13C	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.61 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.776 ppm	internal	direct	0.0
13C	DDS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DDS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1H67, Strand ID: A Detail

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Release date

2001-02-26

Citation 1

Letter to the Editor: 1H, 15N and 13C resonance assignments of the N-terminal region of calponin
Bramham, J., Smith, B.O., Uhrin, D., Barlow, P.N., Winder, S.J.
J. Biomol. NMR (2001), 19, 189-190, PubMed , DOI:

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. calponin CH domain, : 1 : 2 : 133 entities Detail

Interaction partners 1. calponin CH domain, : 1 interactors Detail

Experiments performed 10 experiments Detail