BMRBj - BMREntryMaster

Found 1 Document (1.488 seconds)

BMRB: 4888

2PTK

Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 in high salt solution

Authors

Wang, C., Pawley, N.H., Nicholson, L.K.

Assembly

c-Src SH3 monomer

Entity

1. c-Src SH3 monomer (polymer, Thiol state: not present), 72 monomers, 7979.576 Da Detail

AGGVTTFVAL YDYESRTETD LSFKKGERLQ IVNNTEGDWW LAHSLTTGQT GYIPSNYVAP SDSIQAEGIH RD

Formula weight

7979.576 Da

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 30.9 %, Completeness (bb): 65.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	30.9 % (151 of 488)	19.8 % (81 of 410)	89.7 % (70 of 78)
Backbone	65.3 % (143 of 219)	49.0 % (73 of 149)	100.0 % (70 of 70)
Sidechain	3.0 % (8 of 269)	3.1 % (8 of 261)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 42)	0.0 % (0 of 2)
Methyl	0.0 % (0 of 39)	0.0 % (0 of 39)

1. Src Homology 3 domain

AGGVTTFVAL YDYESRTETD LSFKKGERLQ IVNNTEGDWW LAHSLTTGQT GYIPSNYVAP SDSIQAEGIH RD

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Src Homology 3 domain	[U-N15]	1 mM
2	sodium phosphate		50 mM
3	sodium sulphate		200 mM
4	DTT		10 mM
5	pepstatin		5 mg/mL
6	EDTA		50 uM
7	benzamidine		10 ug/mL
8	chloramphenicol		50 uM

Release date

2002-01-22

Citation

The role of backbone motions in ligand binding to the c-Src SH3 domain
Wang, C., Pawley, N.H., Nicholson, L.K.
J. Mol. Biol. (2001), 313, 873-887, PubMed 11697910 , DOI 10.1006/jmbi.2001.5083 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 4889 released on 2002-01-22
Title Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 complexed with RLP2 ligand in high salt solution

Related entities 1. c-Src SH3 monomer, : 1 : 26 : 234 entities Detail

Interaction partners 1. c-Src SH3 monomer, : 19 interactors Detail

More details for 19 interactors identified by 16 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4888_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4888_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	sh3_unlig	OK	140		97.2 (chain: 1, length: 72)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: sh3_unlig
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 72, Sequence coverage: 97.2%
- Total number of assignments
  - ¹H chemical shifts: 70
  - ¹⁵N chemical shifts: 70
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.488 seconds)

BMRB: 4888

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. c-Src SH3 monomer, : 1 : 26 : 234 entities Detail

Interaction partners 1. c-Src SH3 monomer, : 19 interactors Detail

Experiments performed 3 experiments Detail

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Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail