BMRBj - BMREntryMaster

Found 1 Document (1.643 seconds)

BMRB: 4901

2RUK

1H, 15N, 13C assignments of the N-terminal domain of the human TFIIH p62 subunit

Authors

Gervais, V., Lamour, V., Gaudin, F., Thierry, J., Kieffer, B.

Assembly

p62 N-terminal domain

Entity

1. p62 N-terminal domain (polymer, Thiol state: all free), 110 monomers, 12428.26 Da Detail

GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN

Formula weight

12428.26 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.6 %, Completeness: 78.1 %, Completeness (bb): 77.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.1 % (1034 of 1324)	86.5 % (602 of 696)	63.5 % (324 of 510)	91.5 % (108 of 118)
Backbone	77.8 % (509 of 654)	91.4 % (203 of 222)	63.1 % (205 of 325)	94.4 % (101 of 107)
Sidechain	80.3 % (622 of 775)	84.2 % (399 of 474)	74.5 % (216 of 290)	63.6 % (7 of 11)
Aromatic	43.8 % (35 of 80)	87.5 % (35 of 40)	0.0 % (0 of 39)	0.0 % (0 of 1)
Methyl	97.6 % (121 of 124)	98.4 % (61 of 62)	96.8 % (60 of 62)

1. human TFIIH p62 subunit

GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN

Show sample condition

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

Release date

2001-04-25

Citation 1

Assignment of the 1H, 15N, 13C resonances of the N-terminal domain of the human TFIIH P62 subunit
Gervais, V., Lamour, V., Gaudin, F., Thierry, J., Kieffer, B.
J. Biomol. NMR (2001), 19, 281-282, PubMed 11330817 , Abstract

Show more 2 citaions

Citation 2

Cloning of the 62-kilodalton component of basic transcription factor BTF2
Fischer, L., Gerard, M., Chalut, C., Lutz, Y., Humbert, S., Kanno, M., Chambon, P., Egly, J.M.
Science (1992), 257, 1392-1395, PubMed 1529339 , DOI: , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
Bartels, C., Xia, T., Billeter, M., Guntert, P., Wuthrich, K.
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 2 citaions

Related entities 1. p62 N-terminal domain, : 1 : 4 : 22 : 6 entities Detail

Interaction partners 1. p62 N-terminal domain, : 15 interactors Detail

More details for 15 interactors identified by 12 citations

Experiments performed 13 experiments Detail

1 15N HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

2 HNCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

3 HN(CO)CA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

5 HNCACB

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

6 15N-TOCSY-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

7 15N-NOESY-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

8 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

9 C(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

10 H(CCO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

11 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

12 2D NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

13 2D TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1), Details The isotope labeling is over 98%.

#	Name	Isotope labeling	Type	Concentration
1	human TFIIH p62 subunit	[13C; U-98% 15N]		1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	human TFIIH p62 subunit			2.4 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4901_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4901_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1153		92.7 (chain: 1, length: 110)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 110, Sequence coverage: 92.7%
- Total number of assignments
  - ¹H chemical shifts: 729
  - ¹³C chemical shifts: 316
  - ¹⁵N chemical shifts: 108
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
37	PRO	N	121.57

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	696	607	87.2
¹³C chemical shifts	510	316	62.0
¹⁵N chemical shifts	123	107	87.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	222	204	91.9
¹³C chemical shifts	220	100	45.5
¹⁵N chemical shifts	107	100	93.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	474	403	85.0
¹³C chemical shifts	290	216	74.5
¹⁵N chemical shifts	16	7	43.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	61	93.8
¹³C chemical shifts	65	60	92.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	40	35	87.5
¹³C chemical shifts	39	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords p62, transcription factor, TFIIH, heteronuclear NMR, spectrum analysis, software

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Found 1 Document (1.643 seconds)

BMRB: 4901

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Related entities 1. p62 N-terminal domain, : 1 : 4 : 22 : 6 entities Detail

Interaction partners 1. p62 N-terminal domain, : 15 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail