BMRBj - BMREntryMaster

Found 1 Document (1.39 seconds)

BMRB: 4931

Solution structure of dynein light chain 8 (DLC8) and bim peptide complex

Authors

Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.

Assembly

DYNEIN

Entity

1. DYNEIN light chain 8 (polymer, Thiol state: all free), 89 monomers, 10365.76 × 2 Da Detail

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

2. Bcl-2 (polymer, Thiol state: all free), 9 monomers, 1000.105 × 2 Da Detail

MSCDKSTQT

Total weight

22731.73 Da

Max. entity weight

10365.76 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 72.7 %, Completeness (bb): 74.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.7 % (853 of 1174)	82.2 % (502 of 611)	57.9 % (264 of 456)	81.3 % (87 of 107)
Backbone	74.9 % (439 of 586)	87.9 % (175 of 199)	61.7 % (179 of 290)	87.6 % (85 of 97)
Sidechain	72.7 % (496 of 682)	79.4 % (327 of 412)	64.2 % (167 of 260)	20.0 % (2 of 10)
Aromatic	41.5 % (49 of 118)	76.3 % (45 of 59)	5.2 % (3 of 58)	100.0 % (1 of 1)
Methyl	92.2 % (83 of 90)	93.3 % (42 of 45)	91.1 % (41 of 45)

1. DYNEIN light chain 8

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG

2. Bcl-2

MSCDKSTQT

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	DYNEIN light chain 8	[U-15N; U-13C]		1.5 mM
2	Bcl-2			1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -1.22 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -1.22 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Release date

2001-08-21

Citation

Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain
Fan, J., Zhang, Q., Tochio, H., Li, M., Zhang, M.
J. Mol. Biol. (2001), 306, 97-108, PubMed 11178896 , DOI 10.1006/jmbi.2000.4374 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 4911 released on 2001-08-07
    Title Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain
  BMRB: 4912 released on 2001-08-07
    Title Structural Basis of Diverse Sequence-dependent Target Recognition by the 8kDa Dynein light chain
  Swiss-Prot: P63170 released on 2004-09-27
    Title DYL1_RAT Entity Dynein light chain 1, cytoplasmic

Related entities 1. DYNEIN light chain 8, : 3 : 1 : 98 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4931_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4931_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_set_1	Warning	956		100.0 (chain: 1, length: 89)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: chemical_shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 100.0%
- Total number of assignments
  - ¹⁵N chemical shifts: 89
  - ¹H chemical shifts: 608
  - ¹³C chemical shifts: 259
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
41	HIS	HD1	6.74
55	HIS	HD1	7.26
72	HIS	HD1	7.57

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	99	89	89.9
¹H chemical shifts	562	513	91.3
¹³C chemical shifts	421	259	61.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	88	88	100.0
¹H chemical shifts	181	181	100.0
¹³C chemical shifts	178	89	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	11	1	9.1
¹H chemical shifts	381	332	87.1
¹³C chemical shifts	243	170	70.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	46	100.0
¹³C chemical shifts	46	46	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	1	1	100.0
¹H chemical shifts	59	43	72.9
¹³C chemical shifts	58	0	0.0

Keywords dynein, light chain, DLC8, Bim

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Found 1 Document (1.39 seconds)

BMRB: 4931

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

Related entities 1. DYNEIN light chain 8, : 3 : 1 : 98 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail