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Found 1 Document (1.343 seconds)

BMRB: 4943

9B7F

1H Chemical shift Assignments for Lysozymes marketed by Seikagaku and Sigma companies at pH 3.8, 308K

Authors

Poznanski, J., Georgalis, Y., Wehr, L., Zielenkiewicz, P., Saenger, W.

Assembly

Chicken Egg White Lysozyme

Entity

1. Chicken Egg White Lysozyme (polymer, Thiol state: all disulfide bound), 133 monomers, 14827.54 Da Detail

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRLD RSR

Formula weight

14827.54 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS127:SG
2	disulfide	sing	1:CYS30:SG	1:CYS115:SG
3	disulfide	sing	1:CYS64:SG	1:CYS80:SG
4	disulfide	sing	1:CYS76:SG	1:CYS94:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.7 %, Completeness: 64.9 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	64.9 % (505 of 778)	64.9 % (505 of 778)
Backbone	97.8 % (270 of 276)	97.8 % (270 of 276)
Sidechain	46.8 % (235 of 502)	46.8 % (235 of 502)
Aromatic	9.2 % (6 of 65)	9.2 % (6 of 65)
Methyl	35.6 % (21 of 59)	35.6 % (21 of 59)

1. Chicken Egg White Lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRLD RSR

Show sample condition

Sample

Temperature 308 (±1) K, pH 3.8 (±0.1), Details Lysozyme marketed by Sigma

#	Name	Isotope labeling	Type	Concentration
1	Chicken Egg White Lysozyme			10 mM

Chem. Shift Complete2

Sequence coverage: 96.2 %, Completeness: 63.0 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	63.0 % (980 of 1556)	63.0 % (980 of 1556)
Backbone	97.1 % (536 of 552)	97.1 % (536 of 552)
Sidechain	44.2 % (444 of 1004)	44.2 % (444 of 1004)
Aromatic	9.2 % (12 of 130)	9.2 % (12 of 130)
Methyl	32.2 % (38 of 118)	32.2 % (38 of 118)

1. Chicken Egg White Lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT QATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRLD RSR

Show sample condition

Sample

Temperature 308 (±1) K, pH 3.8 (±0.1), Details Lysozyme marketed by Seikagaku

#	Name	Isotope labeling	Type	Concentration
2	Chicken Egg White Lysozyme			10 mM

Release date

2003-05-20

Citation 1

Comparison of two different lysozyme types under native and crystallization conditions using two-dimensional NMR and dynamic light scattering
Poznanski, J., Georgalis, Y., Wehr, L., Zielenkiewicz, P., Saenger, W.
Biophys. Chem. (2003), 104, 605-616, PubMed 12914906 , DOI 10.1016/s0301-4622(03)00061-9 , Abstract

Show more 3 citaions

Citation 2

Sequential 1H NMR assignments and secondary structure of hen egg white lysozyme in solution
Redfield, C., Dobson, C.M.
Biochemistry (1988), 27, 122-136, PubMed 3349024 , DOI 10.1021/bi00401a020 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 4

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Hide non-primary 3 citaions

Related entities 1. Chicken Egg White Lysozyme, : 1 : 105 : 141 entities Detail

Interaction partners 1. Chicken Egg White Lysozyme, : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4943_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:6:CYS:SG	1:127:CYS:SG	unknown	unknown	n/a
1:30:CYS:SG	1:115:CYS:SG	unknown	unknown	n/a
1:64:CYS:SG	1:80:CYS:SG	unknown	unknown	n/a
1:76:CYS:SG	1:94:CYS:SG	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4943_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	Sigma	Warning	482	96.2 (chain: 1, length: 133)
2	Assigned chemical shifts	Seikagaku	Warning	482	96.2 (chain: 1, length: 133)
1	Chemical shift references	chemical_shift_reference	OK	1	No information

Assigned chemical shifts

Saveframe: Sigma
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 133, Sequence coverage: 96.2%
- Total number of assignments
  - ¹H chemical shifts: 482
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: Seikagaku
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 133, Sequence coverage: 96.2%
- Total number of assignments
  - ¹H chemical shifts: 482
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	778	458	58.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	276	266	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	502	192	38.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	12	19.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	778	458	58.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	276	266	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	502	192	38.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	12	19.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	0	0.0

Keywords Lysozyme

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Found 1 Document (1.343 seconds)

BMRB: 4943

Sample

Sample

Related entities 1. Chicken Egg White Lysozyme, : 1 : 105 : 141 entities Detail

Interaction partners 1. Chicken Egg White Lysozyme, : 7 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail