BMRB: 4979

1H and 13C chemical shift assignments for the pheromone Er-23 from Euplotes raikovi

Authors

Zahn, R., Damberger, F.F., Ortenzi, C., Luporini, P., Wuthrich, K.

Assembly

Er-23 pheromone

Entity

1. Er-23 pheromone (polymer, Thiol state: all disulfide bound), 51 monomers, 5104.517 Da Detail

GECEQCFSDG GDCTTCFNNG TGPCANCLAG YPAGCSNSDC TAFLSQCYGG C

Formula weight

5104.517 Da

Entity Connection

disulfide 5 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS3:SG	1:CYS24:SG
2	disulfide	sing	0:CYS6:SG	1:CYS16:SG
3	disulfide	sing	0:CYS13:SG	1:CYS47:SG
4	disulfide	sing	0:CYS27:SG	1:CYS40:SG
5	disulfide	sing	0:CYS35:SG	1:CYS51:SG

Source organism

Euplotes raikovi

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 70.3 %, Completeness (bb): 66.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	70.3 % (303 of 431)	98.8 % (243 of 246)	32.4 % (60 of 185)
Backbone	66.0 % (167 of 253)	99.1 % (108 of 109)	41.0 % (59 of 144)
Sidechain	63.6 % (140 of 220)	98.5 % (135 of 137)	6.0 % (5 of 83)
Aromatic	45.7 % (21 of 46)	91.3 % (21 of 23)	0.0 % (0 of 23)
Methyl	50.0 % (12 of 24)	100.0 % (12 of 12)	0.0 % (0 of 12)

1. Er-23 pheromone

GECEQCFSDG GDCTTCFNNG TGPCANCLAG YPAGCSNSDC TAFLSQCYGG C

Show sample condition

Sample

Temperature 290 (±1) K, pH 4.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Er-23 pheromone			1.0 mM
2	H2O			90 %
3	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1HA8, Strand ID: A Detail

---

Release date

2001-11-08

Citation 1

NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds
Zahn, R., Damberger, F.F., Ortenzi, C., Luporini, P., Wuthrich, K.
J. Mol. Biol. (2001), 313, 923-931, PubMed 11700049 , DOI 10.1006/jmbi.2001.5099 , Abstract

Show more 4 citaions

Citation 2

NMR structure of the pheromone Er-22 from Euplotes raikovi
Liu, A., Luginbuhl, P., Zerbe, O., Ortenzi, C., Luporini, P., Wuthrich, K.
J. Biomol. NMR (2001), 19, 75-78, PubMed 11246857 , DOI 10.2210/pdb1ha8/pdb , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Citation 4

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Citation 5

The new program OPAL for molecular dynamics simulations and energy refinements of biological macromolecules
Luginbuhl, P., Guntert, P., Billeter, M., Wuthrich, K.
J. Biomol. NMR (1996), 8, 136-146, PubMed 8914272 , DOI 10.1007/bf00211160 , Abstract

Hide non-primary 4 citaions

Related entities 1. Er-23 pheromone, : 1 : 1 : 6 entities Detail

Experiments performed 4 experiments Detail