Backbone 1H, 13C, and 15N Chemical Shift Assignments for proIAPP in DPC Micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.4 % (375 of 844) | 29.8 % (132 of 443) | 57.5 % (185 of 322) | 73.4 % (58 of 79) |
Backbone | 71.8 % (306 of 426) | 56.9 % (82 of 144) | 77.9 % (166 of 213) | 84.1 % (58 of 69) |
Sidechain | 25.1 % (122 of 487) | 16.7 % (50 of 299) | 40.4 % (72 of 178) | 0.0 % (0 of 10) |
Aromatic | 0.0 % (0 of 44) | 0.0 % (0 of 22) | 0.0 % (0 of 22) | |
Methyl | 35.9 % (28 of 78) | 33.3 % (13 of 39) | 38.5 % (15 of 39) |
1. entity 1
TPIESHQVEK RKCNTATCAT QRLANFLVHS SNNFGAILSS TNVGSNTYGK RNAVEVLKRE PLNYLPLKKK KDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 7.0, Details proIAPP-K4D_pH7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DPC | natural abundance | 200 mM | |
2 | TRIS | natural abundance | 30 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | D2O | [U-100% 2H] | 10 v/v | |
5 | sodium azide | natural abundance | 0.03 % | |
6 | proIAPP-K4D | [U-100% 13C; U-100% 15N] | 1.0 (±0.2) mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 7.0, Details proIAPP_pH4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | DPC | natural abundance | 200 mM | |
8 | sodium acetate | natural abundance | 30 mM | |
9 | sodium chloride | natural abundance | 50 mM | |
10 | D2O | [U-100% 2H] | 10 v/v | |
11 | sodium azide | natural abundance | 0.03 % | |
12 | proIAPP-K4D | [U-100% 13C; U-100% 15N] | 1.0 (±0.2) mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 66.9 % (1130 of 1688) | 59.1 % (524 of 886) | 74.5 % (480 of 644) | 79.7 % (126 of 158) |
Backbone | 84.6 % (721 of 852) | 76.7 % (221 of 288) | 88.0 % (375 of 426) | 90.6 % (125 of 138) |
Sidechain | 54.4 % (530 of 974) | 50.7 % (303 of 598) | 63.5 % (226 of 356) | 5.0 % (1 of 20) |
Aromatic | 12.5 % (11 of 88) | 25.0 % (11 of 44) | 0.0 % (0 of 44) | |
Methyl | 66.7 % (104 of 156) | 65.4 % (51 of 78) | 67.9 % (53 of 78) |
1. entity 1
TPIESHQVEK RKCNTATCAT QRLANFLVHS SNNFGAILSS TNVGSNTYGK RNAVEVLKRE PLNYLPLKKK KDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 4.5, Details proIAPP-K4D_pH7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DPC | natural abundance | 200 mM | |
2 | TRIS | natural abundance | 30 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | D2O | [U-100% 2H] | 10 v/v | |
5 | sodium azide | natural abundance | 0.03 % | |
6 | proIAPP-K4D | [U-100% 13C; U-100% 15N] | 1.0 (±0.2) mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 4.5, Details proIAPP_pH4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | DPC | natural abundance | 200 mM | |
8 | sodium acetate | natural abundance | 30 mM | |
9 | sodium chloride | natural abundance | 50 mM | |
10 | D2O | [U-100% 2H] | 10 v/v | |
11 | sodium azide | natural abundance | 0.03 % | |
12 | proIAPP-K4D | [U-100% 13C; U-100% 15N] | 1.0 (±0.2) mM |