Resonance assignment of human STIM1 CC1 fragment
GSPEFNRYSK EHMKKMMKDL EGLHRAEQSL HDLQERLHKA QEEHRTVEVE KVHLEKKLRD EINLAKQEAQ RLKELREGTE NERSRQKYAE EELEQVREAL RKAEKELESH SSWYA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 63.9 % (910 of 1425) | 61.9 % (470 of 759) | 65.1 % (345 of 530) | 69.9 % (95 of 136) |
Backbone | 77.9 % (536 of 688) | 76.3 % (177 of 232) | 78.7 % (269 of 342) | 78.9 % (90 of 114) |
Sidechain | 54.7 % (464 of 849) | 55.8 % (294 of 527) | 55.0 % (165 of 300) | 22.7 % (5 of 22) |
Aromatic | 10.8 % (8 of 74) | 18.9 % (7 of 37) | 0.0 % (0 of 36) | 100.0 % (1 of 1) |
Methyl | 58.7 % (54 of 92) | 65.2 % (30 of 46) | 52.2 % (24 of 46) |
1. entity 1
GSPEFNRYSK EHMKKMMKDL EGLHRAEQSL HDLQERLHKA QEEHRTVEVE KVHLEKKLRD EINLAKQEAQ RLKELREGTE NERSRQKYAE EELEQVREAL RKAEKELESH SSWYASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - na MHz Bruker Avance 950 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - na MHz Bruker Avance 950 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - na MHz Bruker Avance 950 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - na MHz Bruker Avance 950 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - na MHz Bruker Avance 950 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance - 700 MHz Bruker Avance III 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance NEO - 700 MHz Bruker Avance NEO 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance NEO - 700 MHz Bruker Avance NEO 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance NEO - 700 MHz Bruker Avance NEO 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance NEO - 700 MHz Bruker Avance NEO 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Bruker Avance NEO - 700 MHz Bruker Avance NEO 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.15 K, pH 7.25, Details pH 7.25
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIS | natural abundance | 20 mM | |
2 | SDS | [U-99% 2H] | 7 mM | |
3 | STIM1 CC1 | [U-95% 13C; U-95% 15N] | 0.3 mM | |
4 | H2O | [U-99% 2H] | 10 % w/v | |
5 | H2O | natural abundance | 90 % w/v |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr50114_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 GSPEFNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREAL |||||| ||||||||||||||||||||| |||||||||||||| | | | ||||||||||||| |||||||||||||||| | ||||| .....NRYSKE....MMKDLEGLHRAEQSLHDLQER..KAQEEHRTVEVEKV..E.K.R..INLAKQEAQRLKE.REGTENERSRQKYAEE.L..VREAL -------110----- RKAEKELESHSSWYA | || | |||| ||| R.AE.E.ESHS.WYA
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
9 | SER | HG | 5.794 |
24 | HIS | HE2 | 9.264 |
85 | ARG | HH21 | 7.243 |
85 | ARG | HH22 | 6.714 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 759 | 460 | 60.6 |
13C chemical shifts | 530 | 340 | 64.2 |
15N chemical shifts | 136 | 94 | 69.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 232 | 174 | 75.0 |
13C chemical shifts | 230 | 177 | 77.0 |
15N chemical shifts | 114 | 89 | 78.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 527 | 286 | 54.3 |
13C chemical shifts | 300 | 163 | 54.3 |
15N chemical shifts | 22 | 5 | 22.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 49 | 30 | 61.2 |
13C chemical shifts | 49 | 24 | 49.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 37 | 7 | 18.9 |
13C chemical shifts | 36 | 0 | 0.0 |
15N chemical shifts | 1 | 1 | 100.0 |