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DnaJB1 J-domain+G/F rich region
Authors
Rosenzweig, R., Abayev-Avraham, M.
Assembly
DnaJB1 J-domain+G/F rich region
Entity
1. DnaJB1 J-domain+G/F rich region (polymer, Thiol state: all free), 109 monomers, 12072.01 Da Detail

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRN


Formula weight
12072.01 Da
Source organism
Escherichia coli
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.2 %, Completeness: 77.6 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All77.6 % (964 of 1243)69.2 % (450 of 650)88.2 % (419 of 475)80.5 % (95 of 118)
Backbone87.1 % (561 of 644)77.0 % (177 of 230)93.2 % (289 of 310)91.3 % (95 of 104)
Sidechain71.1 % (491 of 691)65.0 % (273 of 420)84.8 % (218 of 257) 0.0 % (0 of 14)
Aromatic86.2 % (119 of 138)82.6 % (57 of 69)89.9 % (62 of 69)
Methyl100.0 % (62 of 62)100.0 % (31 of 31)100.0 % (31 of 31)

1. B1GF

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRN

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5


#NameIsotope labelingTypeConcentration
1DnaJB1GF[U-15N; U-13C]4 mM
2HEPESnatural abundance50 mM
3potassium chloridenatural abundance50 mM
4sodium azidenatural abundance0.03 %
5D2O[U-2H]10 %
Sample #2

Solvent system 99.9% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5


#NameIsotope labelingTypeConcentration
6DnaJB1GF[U-15N;U-13C]4 mM
7HEPESnatural abundance50 mM
8potassium chloridenatural abundance50 mM
9sodium azidenatural abundance0.03 %
10D2O[U-2H]99.9 %

Release date
2020-01-26
Citation
HSP40 proteins use class-specific regulation to drive HSP70 functional diversity
Faust, O., Abayev-Avraham, M., Wentink, A., Maurer, M., Nillegoda, N.B., London, N., Bukau, B., Rosenzweig, R.
Nature (2020), 587, 489-494, PubMed 33177718 , DOI 10.1038/s41586-020-2906-4 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 50168 released on 2020-01-26
    Title J-domain region of DnaJA2
  BMRB: 50169 released on 2020-01-26
    Title DnaJB1 J-domain
Related entities 1. DnaJB1 J-domain+G/F rich region, : 1 : 2 : 1 : 140 entities Detail
Interaction partners 1. DnaJB1 J-domain+G/F rich region, : 54 interactors Detail
Experiments performed 11 experiments Detail
Chemical shift validation 3 contents Detail
Keywords Hsp40, Hsp70, DnaJB1, Chaperone