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BMRB: 50167

6Z5N

DnaJB1 J-domain+G/F rich region

Authors

Rosenzweig, R., Abayev-Avraham, M.

Assembly

DnaJB1 J-domain+G/F rich region

Entity

1. DnaJB1 J-domain+G/F rich region (polymer, Thiol state: all free), 109 monomers, 12072.01 Da Detail

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRN

Formula weight

12072.01 Da

Source organism

Escherichia coli

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 77.6 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.6 % (964 of 1243)	69.2 % (450 of 650)	88.2 % (419 of 475)	80.5 % (95 of 118)
Backbone	87.1 % (561 of 644)	77.0 % (177 of 230)	93.2 % (289 of 310)	91.3 % (95 of 104)
Sidechain	71.1 % (491 of 691)	65.0 % (273 of 420)	84.8 % (218 of 257)	0.0 % (0 of 14)
Aromatic	86.2 % (119 of 138)	82.6 % (57 of 69)	89.9 % (62 of 69)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. B1GF

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

Sample #2

Solvent system 99.9% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
6	DnaJB1GF	[U-15N;U-13C]		4 mM
7	HEPES	natural abundance		50 mM
8	potassium chloride	natural abundance		50 mM
9	sodium azide	natural abundance		0.03 %
10	D2O	[U-2H]		99.9 %

Release date

2020-01-26

Citation

HSP40 proteins use class-specific regulation to drive HSP70 functional diversity
Faust, O., Abayev-Avraham, M., Wentink, A., Maurer, M., Nillegoda, N.B., London, N., Bukau, B., Rosenzweig, R.
Nature (2020), 587, 489-494, PubMed 33177718 , DOI 10.1038/s41586-020-2906-4 , Abstract

Related entities 1. DnaJB1 J-domain+G/F rich region, : 1 : 2 : 1 : 140 entities Detail

Interaction partners 1. DnaJB1 J-domain+G/F rich region, : 54 interactors Detail

More details for 54 interactors identified by 29 citations

Experiments performed 11 experiments Detail

1 3D HNCACB

Instrument

Bruker Avance - 600 MHz 600 MHz Bruker spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz 600 MHz Bruker spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

3 3D HCACO

Instrument

Bruker Avance - 600 MHz 600 MHz Bruker spectrometer

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

4 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

5 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

6 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

8 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

9 2D HMQC_Methyl

Instrument

Bruker Avance - na MHz 1GHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

10 2D (HB)CB(CGCD)HD

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

11 2D (HB)CB(CGCDCE)HE

Instrument

Bruker Avance - 800 MHz 800 MHz Bruker spectrometer equipped with triple resonance single (z) gradient cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DnaJB1GF	[U-15N; U-13C]		4 mM
2	HEPES	natural abundance		50 mM
3	potassium chloride	natural abundance		50 mM
4	sodium azide	natural abundance		0.03 %
5	D2O	[U-2H]		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50167_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50167_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1_B1GF_BMRB	Warning	1022		96.3 (chain: 1, length: 109)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1_B1GF_BMRB
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 109, Sequence coverage: 96.3%
- Total number of assignments
  - ¹H chemical shifts: 515
  - ¹³C chemical shifts: 414
  - ¹⁵N chemical shifts: 93
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Hsp40, Hsp70, DnaJB1, Chaperone