BMRBj - BMREntryMaster

Found 1 Document (2.671 seconds)

BMRB: 50169

6Z5N

DnaJB1 J-domain

Authors

Rosenzweig, R., Abayev-Avraham, M.

Assembly

DnaJB1 J-domain

Entity

1. DnaJB1 J-domain (polymer, Thiol state: all free), 71 monomers, 8338.106 Da Detail

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG L

Formula weight

8338.106 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 27.3 %, Completeness (bb): 62.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	27.3 % (145 of 532)	17.4 % (79 of 453)	83.5 % (66 of 79)
Backbone	62.9 % (134 of 213)	46.9 % (68 of 145)	97.1 % (66 of 68)
Sidechain	3.4 % (11 of 319)	3.6 % (11 of 308)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 36)	0.0 % (0 of 36)
Methyl	7.7 % (2 of 26)	7.7 % (2 of 26)

1. J-domain of DnaJB1

GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG L

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 2mM 15N, 13C-DnaJB1 J-domain, in 50mM HEPES buffer pH 7.5

#	Name	Isotope labeling	Concentration
1	DnaJB1	[U-15N; U-13C]	2 mM
2	HEPES	natural abundance	50 mM
3	potassium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.03 %
5	D2O	[U-2H]	10 %

Release date

2020-01-26

Citation

HSP40 proteins use class-specific regulation to drive HSP70 functional diversity
Faust, O., Abayev-Avraham, M., Wentink, A., Maurer, M., Nillegoda, N.B., London, N., Bukau, B., Rosenzweig, R.
Nature (2020), 587, 489-494, PubMed 33177718 , DOI 10.1038/s41586-020-2906-4 , Abstract

Related entities 1. DnaJB1 J-domain, : 1 : 4 : 3 : 4 : 273 entities Detail

Interaction partners 1. DnaJB1 J-domain, : 54 interactors Detail

More details for 54 interactors identified by 29 citations

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50169_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50169_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1_B1JD_BMRB	Warning	132		93.0 (chain: 1, length: 71)
1	Chemical shift references	chem_shift_reference_1_Reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1_B1JD_BMRB
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 71, Sequence coverage: 93.0%
- Total number of assignments
  - ¹H chemical shifts: 66
  - ¹⁵N chemical shifts: 66
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	453	66	14.6
¹⁵N chemical shifts	79	66	83.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	145	66	45.5
¹⁵N chemical shifts	68	66	97.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	308	0	0.0
¹⁵N chemical shifts	11	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	36	0	0.0

Keywords Hsp40, Hsp70, DnaJB1, Chaperone

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Found 1 Document (2.671 seconds)

BMRB: 50169

Sample

Related entities 1. DnaJB1 J-domain, : 1 : 4 : 3 : 4 : 273 entities Detail

Interaction partners 1. DnaJB1 J-domain, : 54 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail