Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus
MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMA
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.6 % (1459 of 1542) | 95.2 % (755 of 793) | 94.0 % (569 of 605) | 93.8 % (135 of 144) |
Backbone | 95.4 % (761 of 798) | 95.5 % (256 of 268) | 95.5 % (383 of 401) | 94.6 % (122 of 129) |
Sidechain | 94.1 % (823 of 875) | 95.0 % (499 of 525) | 92.8 % (311 of 335) | 86.7 % (13 of 15) |
Aromatic | 86.7 % (78 of 90) | 88.9 % (40 of 45) | 84.1 % (37 of 44) | 100.0 % (1 of 1) |
Methyl | 97.3 % (181 of 186) | 98.9 % (92 of 93) | 95.7 % (89 of 93) |
1. KaiA
MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMATemperature 298 (±0.05) K, pH 6.4 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | KaiA | [U-99% 13C; U-99% 15N] | 2.9 ~ 3.4 mM |