BMRB: 5032

1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX

Authors

Weigelt, J., Climent, I., Dahlman-Wright, K., Wikstrom, M.

Assembly

AFX in complex with DNA

Entity

1. AFX DNA binding domain (polymer, Thiol state: not present), 108 monomers, 12330.65 Da Detail

MRGSHHHHHH GSRRNAWGNQ SYAELISQAI ESAPEKRLTL AQIYEWMVRT VPYFKDKGDS NSSAGWKNSI RHNLSLHSKF IKVHNEATGK SSWWMLNPEG GKSGKAPR

2. Insulin response element from IGFBP-1 promoter (polymer, Thiol state: not present), 16 monomers, 4925.197 Da Detail

CAAGCAAAAC AAACCA

3. Insulin response element from IGFBP-1 promoter (polymer, Thiol state: not present), 16 monomers, 4995.153 Da Detail

TGGTTTGTTT TGCTTG

Total weight

22251.0 Da

Max. entity weight

12330.65 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 60.0 %, Completeness: 22.9 %, Completeness (bb): 36.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	1H	13C	15N
All	22.9 % (363 of 1584)	11.2 % (110 of 979)	36.4 % (177 of 486)	63.9 % (76 of 119)
Backbone	36.8 % (318 of 864)	18.7 % (83 of 445)	50.5 % (159 of 315)	73.1 % (76 of 104)
Sidechain	13.8 % (113 of 819)	5.1 % (27 of 534)	31.9 % (86 of 270)	0.0 % (0 of 15)
Aromatic	0.0 % (0 of 204)	0.0 % (0 of 134)	0.0 % (0 of 65)	0.0 % (0 of 5)
Methyl	17.0 % (15 of 88)	6.1 % (3 of 49)	30.8 % (12 of 39)

1. AFX DNA binding domain

MRGSHHHHHH GSRRNAWGNQ SYAELISQAI ESAPEKRLTL AQIYEWMVRT VPYFKDKGDS NSSAGWKNSI RHNLSLHSKF IKVHNEATGK SSWWMLNPEG GKSGKAPR

2. Insulin response element from IGFBP-1 promoter

CAAGCAAAAC AAACCA

3. Insulin response element from IGFBP-1 promoter

TGGTTTGTTT TGCTTG

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.4 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	AFX DNA binding domain	[U-13C; U-15N; U-90% 2H]		0.5 mM
2	Insulin response element from IGFBP-1 promoter			0.5 mM
3	Insulin response element from IGFBP-1 promoter			0.5 mM
4	sodium phospate			20 mM
5	NaCl			50 mM
6	MgCl2			2 mM
7	NaN3			0.02 % w/v

Hide sample codition

LACS Plot; CA

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	proton	4.773 ppm	internal	direct	0.0
15N	H2O	proton	4.773 ppm	internal	indirect	0.1013291
13C	H2O	proton	4.773 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	proton	4.773 ppm	internal	direct	0.0
15N	H2O	proton	4.773 ppm	internal	indirect	0.1013291
13C	H2O	proton	4.773 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

Release date

2001-06-04

Citation

1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX
Weigelt, J., Climent, I., Dahlman-Wright, K., Wikstrom, M.
J. Biomol. NMR (2000), 17, 181-182, PubMed 10921784 , Abstract

Related entities 1. AFX DNA binding domain, : 2 : 77 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 5 contents Detail