1H, 13C and 15N chemical shift assignment of an intein protein from a cyanobacterium Spirulina platensis
EALTGDALIL SDRGWLRIDD PTLQECRVLS YNESTQQWEW QQVLRWLDQG VRETWKIKTF QTEIKCTGNH LIRTDKGWIK AANITPKMKI LSPEIDAAVK TALQDVESIE KLGVNHVYDI EVEHNHNFVA NGLLVHN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.5 % (1537 of 1643) | 92.7 % (786 of 848) | 93.8 % (594 of 633) | 96.9 % (157 of 162) |
Backbone | 98.7 % (805 of 816) | 97.5 % (271 of 278) | 99.3 % (401 of 404) | 99.3 % (133 of 134) |
Sidechain | 89.6 % (857 of 957) | 89.8 % (512 of 570) | 89.4 % (321 of 359) | 85.7 % (24 of 28) |
Aromatic | 52.3 % (67 of 128) | 56.3 % (36 of 64) | 43.1 % (25 of 58) | 100.0 % (6 of 6) |
Methyl | 99.4 % (175 of 176) | 98.9 % (87 of 88) | 100.0 % (88 of 88) |
1. entity 1
EALTGDALIL SDRGWLRIDD PTLQECRVLS YNESTQQWEW QQVLRWLDQG VRETWKIKTF QTEIKCTGNH LIRTDKGWIK AANITPKMKI LSPEIDAAVK TALQDVESIE KLGVNHVYDI EVEHNHNFVA NGLLVHNSolvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Bruker AVANCE III - 600 MHz
State isotropic, Solvent system 93% H2O/7% D2O, Temperature 303 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Spl DnaX mini-intein | [U-100% 13C; U-100% 15N] | 0.65 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr50361_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 EALTGDALILSDRGWLRIDDPTLQECRVLSYNESTQQWEWQQVLRWLDQGVRETWKIKTFQTEIKCTGNHLIRTDKGWIKAANITPKMKILSPEIDAAVK |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| EALTGDALILSDRGWLRIDDPTLQECRVLSYNESTQQWEWQQVLRWLDQGVRETWKIKTFQTEIKCTGNHLIRTDKGWIKAANITPKMKILSPEIDAAVK -------110-------120-------130------- TALQDVESIEKLGVNHVYDIEVEHNHNFVANGLLVHN ||||||||||||||||||||||||||||||||||||| TALQDVESIEKLGVNHVYDIEVEHNHNFVANGLLVHN
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 848 | 790 | 93.2 |
13C chemical shifts | 633 | 594 | 93.8 |
15N chemical shifts | 162 | 157 | 96.9 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 278 | 278 | 100.0 |
13C chemical shifts | 274 | 274 | 100.0 |
15N chemical shifts | 134 | 133 | 99.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 570 | 512 | 89.8 |
13C chemical shifts | 359 | 320 | 89.1 |
15N chemical shifts | 28 | 24 | 85.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 89 | 89 | 100.0 |
13C chemical shifts | 89 | 89 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 64 | 36 | 56.2 |
13C chemical shifts | 58 | 25 | 43.1 |
15N chemical shifts | 6 | 6 | 100.0 |