Solution structure and backbone dynamics of human DNA ligase IIIalpha BRCT domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.9 % (792 of 1030) | 74.5 % (398 of 534) | 76.4 % (311 of 407) | 93.3 % (83 of 89) |
Backbone | 93.2 % (481 of 516) | 96.6 % (168 of 174) | 88.8 % (231 of 260) | 100.0 % (82 of 82) |
Sidechain | 64.7 % (387 of 598) | 63.9 % (230 of 360) | 67.5 % (156 of 231) | 14.3 % (1 of 7) |
Aromatic | 1.1 % (1 of 94) | 2.1 % (1 of 47) | 0.0 % (0 of 45) | 0.0 % (0 of 2) |
Methyl | 95.1 % (97 of 102) | 98.0 % (50 of 51) | 92.2 % (47 of 51) |
1. DNA Ligase III
GSADETLCQT KVLLDIFTGV RLYLPPSTPD FSRLRRYFVA FDGDLVQEFD MTSATHVLGS RDKNPAAQQV SPEWIWACIR KRRLVAPCPressure 1 atm, Temperature 288 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA Ligase III | [U-13C; U-15N] | 1.0 mM | |
2 | NaH2PO4 | 50 mM | ||
3 | NaCl | 150 mM | ||
4 | DTT | [U-2H] | 25 mM | |
5 | H2O | 90.0 ~ 95.0 % | ||
6 | D2O | 5.0 ~ 10.0 % |