EmrE S64V Mutant Bound to tetra(4-fluorophenyl)phosphonium at pH 5.8
MNPYIYLGGA ILAEVIGTTL MKFSEGFTRL WPSVGTIICY CASFWLLAQT LAYIPTGIAY AIWVGVGIVL ISLLSWGFFG QRLDLPAIIG MMLICAGVLI INLLSRSTPH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 34.8 % (452 of 1297) | 13.2 % (86 of 653) | 53.8 % (284 of 528) | 70.7 % (82 of 116) |
Backbone | 62.5 % (406 of 650) | 36.1 % (82 of 227) | 76.4 % (243 of 318) | 77.1 % (81 of 105) |
Sidechain | 14.0 % (104 of 745) | 0.9 % (4 of 426) | 32.1 % (99 of 308) | 9.1 % (1 of 11) |
Aromatic | 3.5 % (5 of 142) | 1.4 % (1 of 71) | 4.5 % (3 of 67) | 25.0 % (1 of 4) |
Methyl | 14.4 % (26 of 180) | 2.2 % (2 of 90) | 26.7 % (24 of 90) |
1. entity 1
MNPYIYLGGA ILAEVIGTTL MKFSEGFTRL WPSVGTIICY CASFWLLAQT LAYIPTGIAY AIWVGVGIVL ISLLSWGFFG QRLDLPAIIG MMLICAGVLI INLLSRSTPHSolvent system aqueous buffer, Pressure 1 atm, Temperature 285 K, pH 5.8, Details U-C,N-labelled S64V EmrE, with F4-TPP in DMPC bilayers at 1:25 P:L ratio.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EmrE-S64V | [U-99% 13C; U-99% 15N] | 0.26 mg/uL | |
2 | F4TPP | natural abundance | 9.5 ug/uL | |
3 | DMPC | natural abundance | 0.41 mg/uL | |
4 | MES | natural abundance | 50 mM | |
5 | sodium chloride | natural abundance | 20 mM |
Solvent system aqueous buffer, Pressure 1 atm, Temperature 285 K, pH 5.8, Details U-C,D,N-labelled S64V EmrE, with F4-TPP in d54-DMPC bilayers at 1:25 P:L ratio.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | EmrE-S64V | [U-13C; U-15N; U-2H] | 0.27 mg/uL | |
7 | F4TPP | natural abundance | 9.5 ug/uL | |
8 | d54-DMPC | [U-99% 2H] | 0.44 mg/uL | |
9 | MES | natural abundance | 50 mM | |
10 | sodium chloride | natural abundance | 20 mM |
Solvent system aqueous buffer, Pressure 1 atm, Temperature 285 K, pH 5.8, Details U-C,N-labelled S64V EmrE, with F4-TPP in DMPC bilayers at 1:50 P:L ratio.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | EmrE-S64V | [U-99% 15N] | 0.13 mg/uL | |
12 | F4TPP | natural abundance | 5.4 ug/uL | |
13 | DMPC | natural abundance | 0.46 mg/uL | |
14 | MES | natural abundance | 50 mM | |
15 | sodium chloride | natural abundance | 20 mM |