BMRBj - BMREntryMaster

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BMRB: 50469

A28

Authors

Assembly

A28

Entity

1. A28 (polymer, Thiol state: all disulfide bound), 109 monomers, 12152.60 Da Detail

ATHAAFEYSK SIGGTPALDR RVQDVNDTIS DVKQKWRCVV YPGNGFVSAS IFGFQAEVGP NNTRSIRKFN TMQQCIDFTF SDVININIYN PCVVPNINNA ECQFLKSVL

Formula weight

12152.6 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.1 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1223 of 1272)	95.9 % (628 of 655)	96.5 % (473 of 490)	96.1 % (122 of 127)
Backbone	99.1 % (638 of 644)	98.6 % (216 of 219)	99.1 % (318 of 321)	100.0 % (104 of 104)
Sidechain	94.0 % (687 of 731)	94.5 % (412 of 436)	94.5 % (257 of 272)	78.3 % (18 of 23)
Aromatic	82.5 % (99 of 120)	83.3 % (50 of 60)	83.1 % (49 of 59)	0.0 % (0 of 1)
Methyl	95.8 % (113 of 118)	94.9 % (56 of 59)	96.6 % (57 of 59)

1. entity 1

ATHAAFEYSK SIGGTPALDR RVQDVNDTIS DVKQKWRCVV YPGNGFVSAS IFGFQAEVGP NNTRSIRKFN TMQQCIDFTF SDVININIYN PCVVPNINNA ECQFLKSVL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

Release date

2020-09-13

Citation

NMR assignments of vaccinia virus protein A28: an entry-fusion complex component
Wu, D., Lou, Y.C., Chang, W., Tzou, D.M.
Biomol. NMR Assign. (2021), 15, 117-120, PubMed 33398629 , DOI 10.1007/s12104-020-09993-0 , Abstract

Related entities 1. A28, : 1 : 15 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

3 3D CBCANH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

5 3D HNCO

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

6 3D HN(CA)CO

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

8 3D C(CO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

9 3D H(CCO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

10 3D HBHA(CO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

13 2D HBCBCGCDCEHE

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

14 2D HBCBCGCDHD

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	A28	[U-100% 13C; U-100% 15N]		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50469_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50469_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1319		99.1 (chain: 1, length: 109)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 109, Sequence coverage: 99.1%
- Total number of assignments
  - ¹³C chemical shifts: 471
  - ¹H chemical shifts: 732
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1