BMRBj - BMREntryMaster

Found 1 Document (2.418 seconds)

BMRB: 5048

1KGL

1H and 15N assignments of rat apo cellular retinol-binding protein type I (CRBP-I)

Authors

Franzoni, L., Luecke, C., Perez, C., Cavazzini, D., Rademacher, M., Ludwig, C., Spisni, A., Rossi, G., Rueterjans, H.

Assembly

cellular retinol-binding protein type I

Entity

1. cellular retinol-binding protein type I (polymer, Thiol state: all free), 135 monomers, 15833.88 Da Detail

MPVDFNGYWK MLSNENFEEY LRALDVNVAL RKIANLLKPD KEIVQDGDHM IIRTLSTFRN YIMDFQVGKE FEEDLTGIDD RKCMTTVSWD GDKLQCVQKG EKEGRGWTQW IEGDELHLEM RAEGVTCKQV FKKVH

Formula weight

15833.88 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS COMBINED WITH SIMULATED ANNEALING FOLLOWED BY ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 96.8 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	96.8 % (972 of 1004)	96.8 % (828 of 855)	96.6 % (144 of 149)
Backbone	96.4 % (396 of 411)	96.4 % (268 of 278)	96.2 % (128 of 133)
Sidechain	97.1 % (576 of 593)	97.1 % (560 of 577)	100.0 % (16 of 16)
Aromatic	100.0 % (76 of 76)	100.0 % (72 of 72)	100.0 % (4 of 4)
Methyl	95.5 % (64 of 67)	95.5 % (64 of 67)

1. cellular retinol-binding protein type I

MPVDFNGYWK MLSNENFEEY LRALDVNVAL RKIANLLKPD KEIVQDGDHM IIRTLSTFRN YIMDFQVGKE FEEDLTGIDD RKCMTTVSWD GDKLQCVQKG EKEGRGWTQW IEGDELHLEM RAEGVTCKQV FKKVH

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	cellular retinol-binding protein type I			1.6 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	cellular retinol-binding protein type I	[U-15N]		1.6 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1JBH, Strand ID: A Detail

Release date

2002-06-12

Citation

Structure and backbone dynamics of Apo- and holo-cellular retinol-binding protein in solution
Franzoni, L., Luecke, C., Perez, C., Cavazzini, D., Rademacher, M., Ludwig, C., Spisni, A., Rossi, G., Rueterjans, H.
J. Biol. Chem. (2002), 277, 21983-21997, PubMed 11934897 , DOI 10.1074/jbc.M201994200 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 5319 released on 2002-06-12
    Title 1H and 15N assignments of rat holo cellular retinol-binding protein type I (CRBP-I)
  BMRB: 5330 released on 2002-06-12
    Title Backbone relaxation data of rat apo cellular retinol-binding protein type I (CRBP-I)
  BMRB: 5331 released on 2002-06-12
    Title Backbone relaxation data of rat holo cellular retinol-binding protein type I (CRBP-I)

Related entities 1. cellular retinol-binding protein type I, : 1 : 3 : 27 : 214 entities Detail

Interaction partners 1. cellular retinol-binding protein type I, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5048_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5048_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_1	Warning	1122		97.8 (chain: 1, length: 135)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: shift_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 135, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 973
  - ¹⁵N chemical shifts: 149
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
41	LYS	HZ1	7.39
41	LYS	HZ2	7.39
41	LYS	HZ3	7.39
41	LYS	NZ	31.0
83	CYS	HG	1.17
96	CYS	HG	0.25
105	ARG	HH21	6.64
105	ARG	HH22	6.64
105	ARG	NH2	71.4
108	THR	HG1	6.26
127	CYS	HG	2.14

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	855	826	96.6
¹⁵N chemical shifts	156	147	94.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	278	265	95.3
¹⁵N chemical shifts	133	125	94.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	577	561	97.2
¹⁵N chemical shifts	23	22	95.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	73	69	94.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	72	100.0
¹⁵N chemical shifts	4	4	100.0

Keywords beta barrel, lipid binding protein, retinoid carrier, apo form, 15N isotope enrichment

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Found 1 Document (2.418 seconds)

BMRB: 5048

Sample #1

Sample #2

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. cellular retinol-binding protein type I, : 1 : 3 : 27 : 214 entities Detail

Interaction partners 1. cellular retinol-binding protein type I, : 2 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail