BMRBj - BMREntryMaster

Found 1 Document (0.446 seconds)

BMRB: 50523

7WCG

SsoSSB 1-114

Authors

Yang, M., Park, C., Lee, W.

Assembly

SsoSSB

Entity

1. SsoSSB (polymer, Thiol state: not present), 129 monomers, 14141.55 Da Detail

MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAENLYFQ SGSHHHHHH

Formula weight

14141.55 Da

Source organism

Saccharolobus solfataricus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 83.7 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.7 % (1228 of 1467)	82.5 % (627 of 760)	86.5 % (485 of 561)	79.5 % (116 of 146)
Backbone	93.2 % (714 of 766)	93.2 % (247 of 265)	93.4 % (351 of 376)	92.8 % (116 of 125)
Sidechain	76.2 % (624 of 819)	76.8 % (380 of 495)	80.5 % (244 of 303)	0.0 % (0 of 21)
Aromatic	0.0 % (0 of 90)	0.0 % (0 of 45)	0.0 % (0 of 43)	0.0 % (0 of 2)
Methyl	95.6 % (130 of 136)	95.6 % (65 of 68)	95.6 % (65 of 68)

1. entity 1

MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAENLYFQ SGSHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	SsoSSB	[U-100% 13C; U-100% 15N]		1.2 mM

Release date

2020-10-19

Citation

Resonance assignments and secondary structure of thermophile single-stranded DNA binding protein from Sulfolobus solfataricus at 323K
Yang, M.J., Lee, W., Park, C.J.
Biomol. NMR Assign. (2021), 15, 159-164, PubMed 33405014 , DOI 10.1007/s12104-020-09999-8 , Abstract

Related entities 1. SsoSSB, : 1 : 3 : 14 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50523_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50523_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1355		93.8 (chain: 1, length: 129)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 129, Sequence coverage: 93.8%
- Total number of assignments
  - ¹H chemical shifts: 756
  - ¹³C chemical shifts: 483
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	495	378	76.4
¹³C chemical shifts	303	242	79.9
¹⁵N chemical shifts	21	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	66	94.3
¹³C chemical shifts	70	64	91.4

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Found 1 Document (0.446 seconds)

BMRB: 50523

Sample

Related entities 1. SsoSSB, : 1 : 3 : 14 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	760	624	82.1
¹³C chemical shifts	561	483	86.1
¹⁵N chemical shifts	146	116	79.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	265	246	92.8
¹³C chemical shifts	258	241	93.4
¹⁵N chemical shifts	125	116	92.8