Backbone 1H, 13C, and 15N Chemical Shift Assignments for c-FLIPs_C8-1a compex
MGRSDSAEVI HQVEEALDTD EKEMLLFLCR DVAIDVVPPN VRDLLDILRE RGKLSVGDLA ELLYRVRRFD LLKRILKMDR KAVETHLLRN PHLVSDYRVL MAEIGEDLDK SDVSSLIGLM KDYMGRGKIS KEKSFLDLVV ELEKLNLVAP DQLDLLEKCL KNIHRIDLKT KIQKYKQLEH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.9 % (1950 of 2352) | 82.7 % (1013 of 1225) | 82.7 % (756 of 914) | 85.0 % (181 of 213) |
Backbone | 92.8 % (1067 of 1150) | 93.1 % (362 of 389) | 92.5 % (529 of 572) | 93.1 % (176 of 189) |
Sidechain | 76.4 % (1060 of 1388) | 77.9 % (651 of 836) | 76.5 % (404 of 528) | 20.8 % (5 of 24) |
Aromatic | 11.7 % (14 of 120) | 11.7 % (7 of 60) | 11.7 % (7 of 60) | |
Methyl | 94.8 % (239 of 252) | 96.0 % (121 of 126) | 93.7 % (118 of 126) |
1. entity 1
MGRSDSAEVI HQVEEALDTD EKEMLLFLCR DVAIDVVPPN VRDLLDILRE RGKLSVGDLA ELLYRVRRFD LLKRILKMDR KAVETHLLRN PHLVSDYRVL MAEIGEDLDK SDVSSLIGLM KDYMGRGKIS KEKSFLDLVV ELEKLNLVAP DQLDLLEKCL KNIHRIDLKT KIQKYKQLEH HHHHH2. entity 2
DFSRNLYDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details c-FLIPs protein binding a peptide C8-1a, and complex NMR data of c-FLIPs_C8-1a.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLIPs | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | C8-1a | natural abundance | 4.2 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | C8-1a | natural abundance | 10 mM | |
4 | c-FLIPs | [U-100% 15N] | 0.6 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details c-FLIPs protein binding a peptide C8-1a, and complex NMR data of c-FLIPs_C8-1a.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLIPs | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | C8-1a | natural abundance | 4.2 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details c-FLIPs protein binding a peptide C8-1a, and complex NMR data of c-FLIPs_C8-1a.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLIPs | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | C8-1a | natural abundance | 4.2 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details c-FLIPs protein binding a peptide C8-1a, and complex NMR data of c-FLIPs_C8-1a.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLIPs | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | C8-1a | natural abundance | 4.2 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | C8-1a | natural abundance | 10 mM | |
4 | c-FLIPs | [U-100% 15N] | 0.6 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | C8-1a | natural abundance | 10 mM | |
4 | c-FLIPs | [U-100% 15N] | 0.6 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | C8-1a | natural abundance | 10 mM | |
4 | c-FLIPs | [U-100% 15N] | 0.6 mM |
Bruker AVANCE III - 700 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details c-FLIPs protein binding a peptide C8-1a, and complex NMR data of c-FLIPs_C8-1a.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLIPs | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | C8-1a | natural abundance | 4.2 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr50524_3.str
Assigned chemical shifts
-------- DFSRNLYD |||||||| DFSRNLYD
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MGRSDSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVL ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ...SDSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVL --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180----- MAEIGEDLDKSDVSSLIGLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQLEHHHHHH ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MAEIGEDLDKSDVSSLIGLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQLEHH -------110-------120-------130-------140-------150-------160-------170-------180-
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 51 | 45 | 88.2 |
13C chemical shifts | 38 | 26 | 68.4 |
15N chemical shifts | 10 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 16 | 15 | 93.8 |
13C chemical shifts | 24 | 16 | 66.7 |
15N chemical shifts | 8 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 35 | 30 | 85.7 |
13C chemical shifts | 22 | 18 | 81.8 |
15N chemical shifts | 2 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 2 | 2 | 100.0 |
13C chemical shifts | 2 | 2 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 9 | 5 | 55.6 |
13C chemical shifts | 9 | 5 | 55.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1174 | 958 | 81.6 |
13C chemical shifts | 876 | 720 | 82.2 |
15N chemical shifts | 203 | 179 | 88.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 373 | 351 | 94.1 |
13C chemical shifts | 548 | 511 | 93.2 |
15N chemical shifts | 181 | 174 | 96.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 801 | 607 | 75.8 |
13C chemical shifts | 506 | 378 | 74.7 |
15N chemical shifts | 22 | 5 | 22.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 130 | 126 | 96.9 |
13C chemical shifts | 130 | 121 | 93.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 51 | 0 | 0.0 |
13C chemical shifts | 51 | 0 | 0.0 |