ATT an Arabidopsis thaliana Inhibitor of Trypsin and Chymotrypsin: Sequence-Specific Multinuclear Magnetic Resonance Assignments and Secondary Structure
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS11:SG | 1:CYS63:SG |
2 | disulfide | sing | 1:CYS24:SG | 1:CYS48:SG |
3 | disulfide | sing | 1:CYS33:SG | 1:CYS58:SG |
4 | disulfide | sing | 1:CYS37:SG | 1:CYS60:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.0 % (649 of 755) | 86.7 % (345 of 398) | 82.2 % (235 of 286) | 97.2 % (69 of 71) |
Backbone | 95.2 % (381 of 400) | 96.5 % (138 of 143) | 93.3 % (180 of 193) | 98.4 % (63 of 64) |
Sidechain | 78.2 % (322 of 412) | 81.2 % (207 of 255) | 72.7 % (109 of 150) | 85.7 % (6 of 7) |
Aromatic | 16.2 % (11 of 68) | 23.5 % (8 of 34) | 6.1 % (2 of 33) | 100.0 % (1 of 1) |
Methyl | 90.0 % (36 of 40) | 95.0 % (19 of 20) | 85.0 % (17 of 20) |
1. ATT
CPEIEAQGNE CLKEYGGDVG FGFCAPRIFP TICYTRCREN KGAKGGRCRW GQGSNVKCLC DFCGDTPQPressure 1 atm, Temperature 298 (±0.1) K, pH 5.0 (±0.02), Details The protein was released from its n-terminal fusion partner (Snase) by CNBr cleaveage
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ATT | [U-15N] | 1.0 mM | |
2 | sodium acetate | [U-99% 2H] | 50 mM |
Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.0 (±0.02), Details The protein was released from its n-terminal fusion partner (Snase) by CNBr cleaveage
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | ATT | [U-15N; U-13C] | 1.0 mM | |
4 | sodium acetate | [U-99% 2H] | 50 mM |