BMRBj - BMREntryMaster

Found 1 Document (2.066 seconds)

BMRB: 5061

1KN5

1H, 13C, and 15N Chemical Shift Assignments for DNA-binding domain of ADR6

Authors

Tu, X., Wu, J., Xu, Y., Shi, Y.

Assembly

DNA-binding domain of ADR6

Entity

1. DNA-binding domain of ADR6 (polymer, Thiol state: all free), 122 monomers, 14412.42 Da Detail

RGSGSHHHHH HGSNNKQYEL FMKSLIENCK KRNMPLQSIP EIGNRKINLF YLYMLVQKFG GADQVTRTQQ WSMVAQRLQI SDYQQLESIY FRILLPYERH MISQEGIKET QAKRILQPSL IS

Formula weight

14412.42 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 83.3 %, Completeness (bb): 90.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.3 % (1268 of 1523)	84.0 % (678 of 807)	81.1 % (469 of 578)	87.7 % (121 of 138)
Backbone	90.5 % (655 of 724)	90.3 % (223 of 247)	90.3 % (324 of 359)	91.5 % (108 of 118)
Sidechain	78.7 % (719 of 914)	81.3 % (455 of 560)	75.1 % (251 of 334)	65.0 % (13 of 20)
Aromatic	1.6 % (2 of 128)	1.6 % (1 of 64)	0.0 % (0 of 63)	100.0 % (1 of 1)
Methyl	92.2 % (107 of 116)	96.6 % (56 of 58)	87.9 % (51 of 58)

1. DNA-binding domain of ADR6

RGSGSHHHHH HGSNNKQYEL FMKSLIENCK KRNMPLQSIP EIGNRKINLF YLYMLVQKFG GADQVTRTQQ WSMVAQRLQI SDYQQLESIY FRILLPYERH MISQEGIKET QAKRILQPSL IS

Show sample condition

Sample

Temperature 300 (±1) K, pH 4.92 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	DNA-binding domain of ADR6	[U-99% 13C; U-99% 15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Release date

2001-11-06

Citation

Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary structure of ADR6 DNA-binding domain
Tu, X., Wu, J., Xu, Y., Shi, Y.
J. Biomol. NMR (2001), 21, 187-188, PubMed , DOI:

Related entities 1. DNA-binding domain of ADR6, : 1 : 1 : 8 : 6 entities Detail

Interaction partners 1. DNA-binding domain of ADR6, : 27 interactors Detail

More details for 27 interactors identified by 12 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5061_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5061_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1381		91.0 (chain: 1, length: 122)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 122, Sequence coverage: 91.0%
- Total number of assignments
  - ¹H chemical shifts: 800
  - ¹³C chemical shifts: 463
  - ¹⁵N chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.066 seconds)

BMRB: 5061

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. DNA-binding domain of ADR6, : 1 : 1 : 8 : 6 entities Detail

Interaction partners 1. DNA-binding domain of ADR6, : 27 interactors Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	807	674	83.5
¹³C chemical shifts	578	463	80.1
¹⁵N chemical shifts	146	118	80.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	247	223	90.3
¹³C chemical shifts	244	214	87.7
¹⁵N chemical shifts	118	106	89.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	560	451	80.5
¹³C chemical shifts	334	249	74.6
¹⁵N chemical shifts	28	12	42.9