BMRBj - BMREntryMaster

Found 1 Document (1.425 seconds)

BMRB: 5068

1LSG

Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants

Authors

Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.

Assembly

hen egg white lysozyme

Entity

1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14444.20 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Formula weight

14444.2 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS31:SG	1:CYS116:SG
3	disulfide	sing	1:CYS65:SG	1:CYS81:SG
4	disulfide	sing	1:CYS77:SG	1:CYS95:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 88.4 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	88.4 % (671 of 759)	88.4 % (671 of 759)
Backbone	97.4 % (263 of 270)	97.4 % (263 of 270)
Sidechain	83.4 % (408 of 489)	83.4 % (408 of 489)
Aromatic	100.0 % (65 of 65)	100.0 % (65 of 65)
Methyl	96.6 % (57 of 59)	96.6 % (57 of 59)

1. hen egg white lysozyme

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Show sample condition

Sample

Temperature 298 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-15N]		1.0 ~ 1.2 mM

Release date

2002-02-26

Citation

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , Abstract

Related entities 1. hen egg white lysozyme, : 1 : 1 : 104 : 142 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5068_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:7:CYS:SG	1:128:CYS:SG	unknown	unknown	n/a
1:31:CYS:SG	1:116:CYS:SG	unknown	unknown	n/a
1:65:CYS:SG	1:81:CYS:SG	unknown	unknown	n/a
1:77:CYS:SG	1:95:CYS:SG	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5068_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	788		98.5 (chain: 1, length: 130)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 130, Sequence coverage: 98.5%
- Total number of assignments
  - ¹H chemical shifts: 788
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
34	LYS	HZ1	7.33
34	LYS	HZ2	7.33
34	LYS	HZ3	7.33

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	759	669	88.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	270	265	98.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	489	404	82.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	58	93.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	65	100.0

Keywords Disulfide Intermediate, hen Lysozyme, Protein Folding, Two-Disulfide Variant

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Found 1 Document (1.425 seconds)

BMRB: 5068

Sample

Related entities 1. hen egg white lysozyme, : 1 : 1 : 104 : 142 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail