1H, 13C and 15N chemical shift assignments of RNA binding protein RBM3
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.3 % (858 of 950) | 91.0 % (446 of 490) | 88.2 % (322 of 365) | 94.7 % (90 of 95) |
Backbone | 98.6 % (493 of 500) | 98.9 % (173 of 175) | 98.8 % (240 of 243) | 97.6 % (80 of 82) |
Sidechain | 83.4 % (438 of 525) | 86.7 % (273 of 315) | 78.7 % (155 of 197) | 76.9 % (10 of 13) |
Aromatic | 25.6 % (21 of 82) | 24.4 % (10 of 41) | 26.8 % (11 of 41) | |
Methyl | 100.0 % (74 of 74) | 100.0 % (37 of 37) | 100.0 % (37 of 37) |
1. entity 1
MSSEEGKLFV GGLNFNTDEQ ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA SVAMRAMNGE SLDGRQIRVD HAGKSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA Recognition Motif | [U-100% 15N] | 1.0 mM | |
2 | RNA Recognition Motif | [U-100% 13C; U-100% 15N] | 0.6 mM | |
3 | RNA Recognition Motif | [U-10% 13C; U-100% 15N] | 0.7 mM | |
4 | sodium phosphate buffer | natural abundance | 10 mM | |
5 | NaCl | natural abundance | 200 mM |