BMRB: 50804

1H, 13C and 15N chemical shift assignments of RNA binding protein RBM3

Authors

Boral, S., Roy, S., Basak, A.J., Maiti, S., De, S.

Assembly

RNA Recognition Motif

Entity

1. RNA Recognition Motif (polymer, Thiol state: not present), 84 monomers, 9274.149 Da Detail

MSSEEGKLFV GGLNFNTDEQ ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA SVAMRAMNGE SLDGRQIRVD HAGK

Formula weight

9274.149 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.3 %, Completeness (bb): 98.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.3 % (858 of 950)	91.0 % (446 of 490)	88.2 % (322 of 365)	94.7 % (90 of 95)
Backbone	98.6 % (493 of 500)	98.9 % (173 of 175)	98.8 % (240 of 243)	97.6 % (80 of 82)
Sidechain	83.4 % (438 of 525)	86.7 % (273 of 315)	78.7 % (155 of 197)	76.9 % (10 of 13)
Aromatic	25.6 % (21 of 82)	24.4 % (10 of 41)	26.8 % (11 of 41)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. entity 1

MSSEEGKLFV GGLNFNTDEQ ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA SVAMRAMNGE SLDGRQIRVD HAGK

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	RNA Recognition Motif	[U-100% 15N]		1.0 mM
2	RNA Recognition Motif	[U-100% 13C; U-100% 15N]		0.6 mM
3	RNA Recognition Motif	[U-10% 13C; U-100% 15N]		0.7 mM
4	sodium phosphate buffer	natural abundance		10 mM
5	NaCl	natural abundance		200 mM

Hide sample codition

Release date

2021-03-06

Citation

Structural and dynamic studies of the human RNA binding protein RBM3 reveals the molecular basis of its oligomerization and RNA recognition
Roy, S., Boral, S., Maiti, S., Kushwaha, T., Basak, A.J., Lee, W., Basak, A., Gholap, S.L., Inampudi, K.K., De, S.
FEBS J. (2022), 289, 2847-2864, PubMed 34837346 , DOI 10.1111/febs.16301 , Abstract

Related entities 1. RNA Recognition Motif, : 1 : 2 : 4 : 320 entities Detail

Interaction partners 1. RNA Recognition Motif, : 39 interactors Detail

Experiments performed 12 experiments Detail