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BMRB: 50842

7MC2

Solution structure of Miz-1 Zinc fingers 10 to 12

Authors

Boisvert, O.O.B., Lavigne, P.P.L.

Assembly

Miz-1 ZF 10-12

Entity

1. Miz-1 ZF 10-12, entity 1 (polymer, Thiol state: all other bound), 85 monomers, 9763.183 Da Detail

MKPYVCERCG KRFVQSSQLA NHIRHHDNIR PYKCSVCSKA FVNVGDLSKH IIIHTGEKPY LCDKCGRGFN RVDNLRSHVK TVHQG

2. Miz-1 ZF 10-12, entity ZN (non-polymer), 65.409 × 3 Da

Total weight

9959.409 Da

Max. entity weight

9763.183 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS6:SG	2:ZN1:ZN
2	na	sing	1:CYS9:SG	2:ZN1:ZN
3	na	sing	1:HIS22:HD	2:ZN1:ZN
4	na	sing	1:HIS26:HD	2:ZN1:ZN
5	na	sing	1:CYS34:SG	2:ZN1:ZN
6	na	sing	1:CYS37:SG	2:ZN1:ZN
7	na	sing	1:HIS50:HD	2:ZN1:ZN
8	na	sing	1:HIS54:HD	2:ZN1:ZN
9	na	sing	1:CYS62:SG	2:ZN1:ZN
10	na	sing	1:CYS65:SG	2:ZN1:ZN
11	na	sing	1:HIS78:HD	2:ZN1:ZN
12	na	sing	1:HIS83:HD	2:ZN1:ZN

Source organism

Exptl. method

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.9 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (839 of 1012)	80.7 % (427 of 529)	85.0 % (328 of 386)	86.6 % (84 of 97)
Backbone	97.8 % (493 of 504)	97.7 % (169 of 173)	97.6 % (243 of 249)	98.8 % (81 of 82)
Sidechain	72.4 % (425 of 587)	72.5 % (258 of 356)	75.9 % (164 of 216)	20.0 % (3 of 15)
Aromatic	25.6 % (21 of 82)	48.8 % (20 of 41)	2.4 % (1 of 41)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. entity 1

MKPYVCERCG KRFVQSSQLA NHIRHHDNIR PYKCSVCSKA FVNVGDLSKH IIIHTGEKPY LCDKCGRGFN RVDNLRSHVK TVHQG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

Release date

2021-03-22

Citation

Zinc Fingers 10 and 11 of Miz-1 undergo conformational exchange to achieve specific DNA binding
Boisvert, O., Letourneau, D., Delattre, P., Tremblay, C., Jolibois, E., Montagne, M., Lavigne, P.
Structure (2022), 30, 623-636, PubMed 34963061 , DOI 10.1016/j.str.2021.12.001 , Abstract

Related entities 1. Miz-1 ZF 10-12, entity 1, : 1 : 2 : 2291 entities Detail

Interaction partners 1. Miz-1 ZF 10-12, entity 1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

2 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

3 3D CBCACONH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

6 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

8 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Miz-1 ZF 10-12	[U-99% 13C; U-99% 15N]		500 (±0.5) uM
2	ZINC ion	natural abundance		2.0 (±0.1) mM
3	TCEP	natural abundance		2.0 (±0.1) mM
4	Bis-Tris	natural abundance		50 (±0.1) mM
5	potassium chloride	natural abundance		50 (±0.1) mM

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