SH3-PRR2 peptide complex
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 66.9 % (603 of 901) | 84.3 % (397 of 471) | 41.5 % (146 of 352) | 76.9 % (60 of 78) |
Backbone | 70.4 % (307 of 436) | 92.6 % (137 of 148) | 52.3 % (116 of 222) | 81.8 % (54 of 66) |
Sidechain | 65.2 % (349 of 535) | 80.5 % (260 of 323) | 41.5 % (83 of 200) | 50.0 % (6 of 12) |
Aromatic | 67.0 % (63 of 94) | 89.4 % (42 of 47) | 42.2 % (19 of 45) | 100.0 % (2 of 2) |
Methyl | 57.6 % (38 of 66) | 78.8 % (26 of 33) | 36.4 % (12 of 33) |
1. entity 1
GITAIALYDY QAAGDDEISF DPDDIITNIE MIDDGWWRGV CKGRYGLFPA NYVELRQ2. entity 2
TAFQEPPPKP SRPKYRPPPSolvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details Unlabeled proteins in complex in H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cortactin SH3 domain | natural abundance | 0.75 (±0.02) mM | |
2 | Pyk2 PRR2 peptide | natural abundance | 0.75 (±0.02) mM | |
3 | D2O | natural abundance | 5 (±0.1) % | |
4 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
5 | NaN3 | natural abundance | 0.02 (±0.002) % |
Solvent system 100% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details Unlabeled proteins in complex in D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Cortactin SH3 domain | natural abundance | 0.75 (±0.02) mM | |
7 | Pyk2 PRR2 peptide | natural abundance | 0.75 (±0.02) mM | |
8 | D2O | natural abundance | 100 (±0.1) % | |
9 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
10 | NaN3 | natural abundance | 0.02 (±0.002) % |
Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details CN-labeled SH3 complex in H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | Cortactin SH3 domain | [U-98% 13C; U-98% 15N] | 0.45 (±0.02) mM | |
12 | Pyk2 PRR2 peptide | natural abundance | 0.45 (±0.02) mM | |
13 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
14 | NaN3 | natural abundance | 0.02 (±0.002) % |
Solvent system 100% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details CN-labeled SH3 complex in D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | Cortactin SH3 domain | [U-98% 13C; U-98% 15N] | 0.45 (±0.02) mM | |
16 | Pyk2 PRR2 peptide | natural abundance | 0.45 (±0.02) mM | |
17 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
18 | NaN3 | natural abundance | 0.02 (±0.002) % |
Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details DN-labeled SH3 complex in H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | Cortactin SH3 domain | [U-98% 2H; U-98% 15N] | 0.45 (±0.02) mM | |
20 | Pyk2 PRR2 peptide | natural abundance | 0.45 (±0.02) mM | |
21 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
22 | NaN3 | natural abundance | 0.02 (±0.002) % |
Solvent system 95% H2O/5% D2O, Pressure 1 (±0) atm, Temperature 296 (±0.5) K, pH 6.5 (±0.1), Details free PRR2 peptide
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | Pyk2 PRR2 peptide | natural abundance | 0.45 (±0.02) mM | |
24 | NaPi pH 6.5 | natural abundance | 30 (±0.06) mM | |
25 | NaN3 | natural abundance | 0.02 (±0.002) % |