BMRB: 50912

Structure of the transmembrane domain of the CD28 dimer

Authors

Wen, M., Ouyang, B., Wu, H., Cao, R., Wu, B.

Assembly

CD28-TM dimer

Entity

1. CD28-TM dimer (polymer, Thiol state: not present), 41 monomers, 4659.605 × 2 Da Detail

GPSKPFWVLV VVGGVLAFYS LLVTVAFIIF WVRSKRSRLL H

Total weight

9319.21 Da

Max. entity weight

4659.605 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 75.1 %, Completeness (bb): 96.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.1 % (390 of 519)	76.0 % (199 of 262)	71.8 % (153 of 213)	86.4 % (38 of 44)
Backbone	96.7 % (234 of 242)	94.0 % (78 of 83)	98.3 % (118 of 120)	97.4 % (38 of 39)
Sidechain	61.6 % (194 of 315)	67.6 % (121 of 179)	55.7 % (73 of 131)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 76)	0.0 % (0 of 38)	0.0 % (0 of 36)	0.0 % (0 of 2)
Methyl	98.6 % (69 of 70)	100.0 % (35 of 35)	97.1 % (34 of 35)

1. entity 1

GPSKPFWVLV VVGGVLAFYS LLVTVAFIIF WVRSKRSRLL H

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-13C; U-15N; 75%-2H] Transmembrane Domain of CD28, 30 mM DMPC, 60 mM DHPC, 25 mM MES.

#	Name	Isotope labeling	Type	Concentration
1	CD28 TM	[U-13C; U-15N; 75%-2H]		0.6 mM
2	MES	natural abundance		25 mM
3	DMPC	natural abundance		30 mM
4	DHPC	natural abundance		60 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-13C; U-15N] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.

#	Name	Isotope labeling	Type	Concentration
5	CD28 TM	[U-13C; U-15N]		0.6 mM
6	MES	natural abundance		25 mM
7	DMPC	[acyl chain U-2H]		30 mM
8	DHPC	[acyl chain U-2H]		60 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-15N, U-2H] Transmembrane Domain of CD28, 0.6 mM [U-13C] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.

#	Name	Isotope labeling	Type	Concentration
9	CD28 TM	[U-15N; U-2H]		0.6 mM
10	MES	natural abundance		25 mM
11	DMPC	[acyl chain U-2H]		30 mM
12	DHPC	[acyl chain U-2H]		60 mM
13	CD28 TM	[U-13C]		0.6 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.2 mM [U-15N; U-2H; 15%-13C] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.

#	Name	Isotope labeling	Type	Concentration
14	CD28 TM	[U-15N; 15%-13C]		0.2 mM
15	MES	natural abundance		25 mM
16	DMPC	[acyl chain U-2H]		30 mM
17	DHPC	[acyl chain U-2H]		60 mM

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Release date

2021-04-22

Citation

Structural characterization of a dimerization interface in the CD28 transmembrane domain
Wu, H., Cao, R., Wen, M., Xue, H., OuYang, B.
Structure (2022), 30, 803-812, PubMed 35397202 , DOI 10.1016/j.str.2022.03.004 , Abstract

Related entities 1. CD28-TM dimer, : 2 : 19 entities Detail

Interaction partners 1. CD28-TM dimer, : 8 interactors Detail

Experiments performed 10 experiments Detail