Structure of the transmembrane domain of the CD28 dimer
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.1 % (390 of 519) | 76.0 % (199 of 262) | 71.8 % (153 of 213) | 86.4 % (38 of 44) |
Backbone | 96.7 % (234 of 242) | 94.0 % (78 of 83) | 98.3 % (118 of 120) | 97.4 % (38 of 39) |
Sidechain | 61.6 % (194 of 315) | 67.6 % (121 of 179) | 55.7 % (73 of 131) | 0.0 % (0 of 5) |
Aromatic | 0.0 % (0 of 76) | 0.0 % (0 of 38) | 0.0 % (0 of 36) | 0.0 % (0 of 2) |
Methyl | 98.6 % (69 of 70) | 100.0 % (35 of 35) | 97.1 % (34 of 35) |
1. entity 1
GPSKPFWVLV VVGGVLAFYS LLVTVAFIIF WVRSKRSRLL HSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-13C; U-15N; 75%-2H] Transmembrane Domain of CD28, 30 mM DMPC, 60 mM DHPC, 25 mM MES.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CD28 TM | [U-13C; U-15N; 75%-2H] | 0.6 mM | |
2 | MES | natural abundance | 25 mM | |
3 | DMPC | natural abundance | 30 mM | |
4 | DHPC | natural abundance | 60 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-13C; U-15N] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | CD28 TM | [U-13C; U-15N] | 0.6 mM | |
6 | MES | natural abundance | 25 mM | |
7 | DMPC | [acyl chain U-2H] | 30 mM | |
8 | DHPC | [acyl chain U-2H] | 60 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.6 mM [U-15N, U-2H] Transmembrane Domain of CD28, 0.6 mM [U-13C] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | CD28 TM | [U-15N; U-2H] | 0.6 mM | |
10 | MES | natural abundance | 25 mM | |
11 | DMPC | [acyl chain U-2H] | 30 mM | |
12 | DHPC | [acyl chain U-2H] | 60 mM | |
13 | CD28 TM | [U-13C] | 0.6 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0, Details 0.2 mM [U-15N; U-2H; 15%-13C] Transmembrane Domain of CD28, 30 mM [acyl chain U-2H] DMPC, 60 mM [acyl chain U-2H] DHPC, 25 mM MES.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | CD28 TM | [U-15N; 15%-13C] | 0.2 mM | |
15 | MES | natural abundance | 25 mM | |
16 | DMPC | [acyl chain U-2H] | 30 mM | |
17 | DHPC | [acyl chain U-2H] | 60 mM |