BMRBj - BMREntryMaster

Found 1 Document (1.254 seconds)

BMRB: 5093

7AFQ

Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for Ribosome-binding Factor A (RbfA)

Authors

Swapna, G.V.T., Shukla, K., Huang, Y., Ke, H., Xia, B., Inouye, M., Montelione, G.T.

Assembly

RbfADelta25

Entity

1. RbfADelta25 (polymer, Thiol state: not present), 108 monomers, 12272.23 Da Detail

MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMR

Formula weight

12272.23 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 90.2 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1163 of 1289)	89.6 % (606 of 676)	89.4 % (448 of 501)	97.3 % (109 of 112)
Backbone	96.7 % (621 of 642)	97.7 % (215 of 220)	95.9 % (304 of 317)	97.1 % (102 of 105)
Sidechain	85.8 % (642 of 748)	85.7 % (391 of 456)	85.6 % (244 of 285)	100.0 % (7 of 7)
Aromatic	67.6 % (50 of 74)	67.6 % (25 of 37)	67.6 % (25 of 37)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. RbfA

MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK DEDAVKAGIK ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMR

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 5.05 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RbfA	[U-13C; U-15N]		1.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Release date

2002-03-03

Citation 1

Resonance assignments for cold-shock protein ribosome-binding factor A (RbfA) from Escherichia coli
Swapna, G.V.T., Shukla, K., Huang, Y., Ke, H., Xia, B., Inouye, M., Montelione, G.T.
J. Biomol. NMR (2001), 21, 389-390, PubMed 11824762 , DOI: , Abstract

Show more 1 citaion

Citation 2

Automated analysis of protein NMR assignments using methods from artificial intelligence
Zimmerman, D.E., Kulikowski, C.A., Huang, Y., Feng, W., Tashiro, M., Shimotakahara, S., Chien, C., Powers, R., Montelione, G.T.
J. Mol. Biol. (1997), 269, 592-610, PubMed 9217263 , DOI 10.1006/jmbi.1997.1052 , Abstract

Hide non-primary 1 citaion

Related entities 1. RbfADelta25, : 1 : 12 : 4 : 105 entities Detail

Interaction partners 1. RbfADelta25, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 1H-15N HSQC