1H, 13C and 15N Chemical Shift Assignments of the R957C mutant from Arkadia (RNF111) E3 RING domain in solution
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS17:SG | 2:ZN1:ZN |
2 | metal coordination | sing | 1:CYS20:SG | 2:ZN1:ZN |
3 | metal coordination | sing | 1:HIS40:ND1 | 2:ZN1:ZN |
4 | metal coordination | sing | 1:CYS43:SG | 2:ZN1:ZN |
5 | metal coordination | sing | 1:CYS35:SG | 2:ZN1:ZN |
6 | metal coordination | sing | 1:HIS37:ND1 | 2:ZN1:ZN |
7 | metal coordination | sing | 1:CYS54:SG | 2:ZN1:ZN |
8 | metal coordination | sing | 1:CYS57:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.2 % (650 of 791) | 87.4 % (361 of 413) | 73.0 % (222 of 304) | 90.5 % (67 of 74) |
Backbone | 84.6 % (345 of 408) | 98.6 % (136 of 138) | 70.1 % (143 of 204) | 100.0 % (66 of 66) |
Sidechain | 82.6 % (371 of 449) | 81.8 % (225 of 275) | 87.3 % (145 of 166) | 12.5 % (1 of 8) |
Aromatic | 0.0 % (0 of 30) | 0.0 % (0 of 15) | 0.0 % (0 of 14) | 0.0 % (0 of 1) |
Methyl | 94.3 % (66 of 70) | 91.4 % (32 of 35) | 97.1 % (34 of 35) |
1. entity 1
MKQDGEEGTE EDTEEKCTIC LSILEEGEDV RCLPCMHLFH QVCVDQWLIT NKKCPICRVD IEAQLPSESSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | R957C | [U-99% 15N] | 0.4 mM | |
2 | DSS | natural abundance | 0.25 mM | |
3 | potassium phosphate | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | R957C | [U-98% 13C; U-98% 15N] | 0.5 mM | |
5 | DSS | natural abundance | 0.25 mM | |
6 | potassium phosphate | natural abundance | 50 mM |