BMRB: 51048

Backbone chemical shift assignments for the post-fusion six-helix bundle (6HB) state of SARS-CoV-2 S2 protein

Authors

Chiliveri, S., Bax, A.

Assembly

SARS-CoV-2 S2 6HB

Entity

1. SARS-CoV-2 S2 6HB (polymer, Thiol state: not present), 96 monomers, 10277.40 Da Detail

GSWNQKLIAN QFNSAIGKIQ DSLSSTASAL GKLQDVVNQN AQALNTLVKQ SGLVPRGSGG INASVVNIQK EIDRLNEVAK NLNESLIDLQ ELGKYE

Formula weight

10277.4 Da

Source organism

Severe acute respiratory syndrome coronavirus 2

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 34.6 %, Completeness (bb): 65.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.6 % (379 of 1096)	16.7 % (95 of 570)	46.8 % (191 of 408)	78.8 % (93 of 118)
Backbone	65.7 % (377 of 574)	47.7 % (95 of 199)	67.5 % (189 of 280)	97.9 % (93 of 95)
Sidechain	0.8 % (5 of 610)	0.0 % (0 of 371)	2.3 % (5 of 216)	0.0 % (0 of 23)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 15)	0.0 % (0 of 14)	0.0 % (0 of 1)
Methyl	1.6 % (2 of 124)	0.0 % (0 of 62)	3.2 % (2 of 62)

1. entity 1

GSWNQKLIAN QFNSAIGKIQ DSLSSTASAL GKLQDVVNQN AQALNTLVKQ SGLVPRGSGG INASVVNIQK EIDRLNEVAK NLNESLIDLQ ELGKYE

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details 0.4 mM 13C/15N/2H 6HB

#	Name	Isotope labeling	Type	Concentration
1	SARS-CoV-2 S2 6HB	[U-13C; U-15N; U-2H]		0.4 mM
2	sodium phosphate buffer	natural abundance		20 mM
3	sodium chloride	natural abundance		30 mM

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Release date

2021-07-29

Citation

Transient lipid-bound states of spike protein heptad repeats provide insights into SARS-CoV-2 membrane fusion
Chiliveri, S.C., Louis, J.M., Ghirlando, R., Bax, A.
Sci. Adv. (2021), 7, eabk2226-eabk2226, PubMed 34623907 , DOI 10.1126/sciadv.abk2226 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. SARS-CoV-2 S2 6HB, : 54 entities Detail

Experiments performed 4 experiments Detail