BMRB: 51052

Assignment of nsp3a-nucleoprotein complex of SARS-CoV-2

Authors

Mamigonian Bessa, L., Camacho Zarco, A., Guseva, S., Salvi, N., Blackledge, M.

Assembly

SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex

Entity

1. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 1 (polymer, Thiol state: all free), 99 monomers, 11255.55 Da Detail

GRRQGYKSVN ITFELDERID KVLNEKCSAY TVELGTEVNE FACVVADAVI KTLQPVSELL TPLGIDLDEW SMATYYLFDE SGEFKLASHM YCSFYPPDE

2. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 2 (polymer, Thiol state: all free), 114 monomers, 12719.19 Da Detail

GAMAPTKVTF GDDTVIEVQG YKSVNITFEL DERIDKVLNE KCSAYTVELG TEVNEFACVV ADAVIKTLQP VSELLTPLGI DLDEWSMATY YLFDESGEFK LASHMYCSFY PPDE

3. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 3 (polymer, Thiol state: all free), 76 monomers, 8065.958 Da Detail

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMSGK GQQQQGQTVT KKSAAEASKK PRQKRT

4. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 4 (polymer, Thiol state: all free), 76 monomers, 8126.054 Da Detail

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMFGK GQQQQGQTVT KKSAAEASKK PRQKRT

5. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 5 (polymer, Thiol state: all free), 92 monomers, 9601.499 Da Detail

GRRGSRGGSQ ASSRSSSRSR NSSRNSTPGS SRGTSPARMA GNGGDAALAL LLLDRLNQLE SKMSGKGQQQ QGQTVTKKSA AEASKKPRQK RT

Total weight

49768.254 Da

Max. entity weight

12719.19 Da

Source organism

Severe acute respiratory syndrome coronavirus 2

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 23.4 %, Completeness: 14.2 %, Completeness (bb): 16.8 % Detail

Download

• NMR-STAR v3.1 (partial)
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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	14.2 % (733 of 5163)	13.7 % (370 of 2691)	13.3 % (259 of 1953)	20.0 % (104 of 519)
Backbone	16.8 % (455 of 2706)	19.7 % (185 of 937)	12.6 % (168 of 1330)	23.2 % (102 of 439)
Sidechain	12.0 % (346 of 2873)	10.5 % (185 of 1754)	15.4 % (160 of 1039)	1.3 % (1 of 80)
Aromatic	0.4 % (1 of 248)	0.0 % (0 of 124)	0.0 % (0 of 122)	50.0 % (1 of 2)
Methyl	26.1 % (111 of 426)	25.8 % (55 of 213)	26.3 % (56 of 213)

1. entity 1

GRRQGYKSVN ITFELDERID KVLNEKCSAY TVELGTEVNE FACVVADAVI KTLQPVSELL TPLGIDLDEW SMATYYLFDE SGEFKLASHM YCSFYPPDE

2. entity 2

GAMAPTKVTF GDDTVIEVQG YKSVNITFEL DERIDKVLNE KCSAYTVELG TEVNEFACVV ADAVIKTLQP VSELLTPLGI DLDEWSMATY YLFDESGEFK LASHMYCSFY PPDE

3. entity 3

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMSGK GQQQQGQTVT KKSAAEASKK PRQKRT

4. entity 4

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMFGK GQQQQGQTVT KKSAAEASKK PRQKRT

5. entity 5

GRRGSRGGSQ ASSRSSSRSR NSSRNSTPGS SRGTSPARMA GNGGDAALAL LLLDRLNQLE SKMSGKGQQQ QGQTVTKKSA AEASKKPRQK RT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 1-111 2H13C15N + N 175-263 1:2

#	Name	Isotope labeling	Type	Concentration
1	SARS-CoV-2 Nsp3 1-111	[U-13C; U-15N; U-2H]		320 uM
2	SARS-CoV-2 N 175-263	natural abundance		640 uM
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		250 mM
5	DTT	natural abundance		2 mM
6	D2O	[U-99% 2H]		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 175-263 2H13C15N + Nsp3 1-111 1:2

#	Name	Isotope labeling	Type	Concentration
7	SARS-CoV-2 Nucleoprotein 175-263	[U-13C; U-15N; U-2H]		600 uM
8	SARS-CoV-2 Nsp3 1-111	natural abundance		1200 uM
9	sodium phosphate	natural abundance		50 mM
10	sodium chloride	natural abundance		250 mM
11	DTT	natural abundance		2 mM
12	D2O	[U-99% 2H]		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
13	SARS-CoV-2 Nsp3 16-111	[U-99% 13C; U-99% 15N]		200 uM
14	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		200 uM
15	sodium phosphate	natural abundance		50 mM
16	sodium chloride	natural abundance		250 mM
17	DTT	natural abundance		2 mM
18	D2O	[U-99% 2H]		10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
19	SARS-CoV-2 Nsp3 16-111	[U-99% 13C; U-99% 15N]		500 uM
20	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		550 uM
21	sodium phosphate	natural abundance		50 mM
22	sodium chloride	natural abundance		250 mM
23	DTT	natural abundance		2 mM
24	D2O	[U-99% 2H]		10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 191-263 13C15N + Nsp3 16-111 191-263

#	Name	Isotope labeling	Type	Concentration
25	SARS-CoV-2 Nucleoprotein 191-263	[U-99% 13C; U-99% 15N]		500 uM
26	SARS-CoV-2 Nsp3 16-111	natural abundance		550 uM
27	sodium phosphate	natural abundance		50 mM
28	sodium chloride	natural abundance		250 mM
29	DTT	natural abundance		2 mM
30	D2O	[U-99% 2H]		10 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 191-263 S235F 13C15N + Nsp3 16-111 191-263

#	Name	Isotope labeling	Type	Concentration
31	SARS-CoV-2 Nucleoprotein 191-263 S235F	[U-99% 13C; U-99% 15N]		500 uM
32	SARS-CoV-2 Nsp3 16-111	natural abundance		550 uM
33	sodium phosphate	natural abundance		50 mM
34	sodium chloride	natural abundance		250 mM
35	DTT	natural abundance		2 mM
36	D2O	[U-99% 2H]		10 %

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
37	SARS-CoV-2 Nsp3 16-111	natural abundance		500 uM
38	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		500 uM
39	sodium phosphate	natural abundance		50 mM
40	sodium chloride	natural abundance		250 mM
41	DTT	natural abundance		2 mM
42	D2O	[U-99% 2H]		10 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 19.3 %, Completeness: 11.9 %, Completeness (bb): 14.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	11.9 % (1225 of 10326)	11.1 % (599 of 5382)	11.2 % (437 of 3906)	18.2 % (189 of 1038)
Backbone	14.6 % (790 of 5412)	16.5 % (310 of 1874)	11.1 % (295 of 2660)	21.1 % (185 of 878)
Sidechain	9.6 % (549 of 5746)	8.2 % (289 of 3508)	12.4 % (257 of 2078)	1.9 % (3 of 160)
Aromatic	0.2 % (1 of 496)	0.0 % (0 of 248)	0.0 % (0 of 244)	25.0 % (1 of 4)
Methyl	17.4 % (148 of 852)	17.4 % (74 of 426)	17.4 % (74 of 426)

1. entity 1

GRRQGYKSVN ITFELDERID KVLNEKCSAY TVELGTEVNE FACVVADAVI KTLQPVSELL TPLGIDLDEW SMATYYLFDE SGEFKLASHM YCSFYPPDE

2. entity 2

GAMAPTKVTF GDDTVIEVQG YKSVNITFEL DERIDKVLNE KCSAYTVELG TEVNEFACVV ADAVIKTLQP VSELLTPLGI DLDEWSMATY YLFDESGEFK LASHMYCSFY PPDE

3. entity 3

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMSGK GQQQQGQTVT KKSAAEASKK PRQKRT

4. entity 4

GRRRNSSRNS TPGSSRGTSP ARMAGNGGDA ALALLLLDRL NQLESKMFGK GQQQQGQTVT KKSAAEASKK PRQKRT

5. entity 5

GRRGSRGGSQ ASSRSSSRSR NSSRNSTPGS SRGTSPARMA GNGGDAALAL LLLDRLNQLE SKMSGKGQQQ QGQTVTKKSA AEASKKPRQK RT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 1-111 2H13C15N + N 175-263 1:2

#	Name	Isotope labeling	Type	Concentration
1	SARS-CoV-2 Nsp3 1-111	[U-13C; U-15N; U-2H]		320 uM
2	SARS-CoV-2 N 175-263	natural abundance		640 uM
3	sodium phosphate	natural abundance		50 mM
4	sodium chloride	natural abundance		250 mM
5	DTT	natural abundance		2 mM
6	D2O	[U-99% 2H]		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 175-263 2H13C15N + Nsp3 1-111 1:2

#	Name	Isotope labeling	Type	Concentration
7	SARS-CoV-2 Nucleoprotein 175-263	[U-13C; U-15N; U-2H]		600 uM
8	SARS-CoV-2 Nsp3 1-111	natural abundance		1200 uM
9	sodium phosphate	natural abundance		50 mM
10	sodium chloride	natural abundance		250 mM
11	DTT	natural abundance		2 mM
12	D2O	[U-99% 2H]		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
13	SARS-CoV-2 Nsp3 16-111	[U-99% 13C; U-99% 15N]		200 uM
14	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		200 uM
15	sodium phosphate	natural abundance		50 mM
16	sodium chloride	natural abundance		250 mM
17	DTT	natural abundance		2 mM
18	D2O	[U-99% 2H]		10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
19	SARS-CoV-2 Nsp3 16-111	[U-99% 13C; U-99% 15N]		500 uM
20	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		550 uM
21	sodium phosphate	natural abundance		50 mM
22	sodium chloride	natural abundance		250 mM
23	DTT	natural abundance		2 mM
24	D2O	[U-99% 2H]		10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 191-263 13C15N + Nsp3 16-111 191-263

#	Name	Isotope labeling	Type	Concentration
25	SARS-CoV-2 Nucleoprotein 191-263	[U-99% 13C; U-99% 15N]		500 uM
26	SARS-CoV-2 Nsp3 16-111	natural abundance		550 uM
27	sodium phosphate	natural abundance		50 mM
28	sodium chloride	natural abundance		250 mM
29	DTT	natural abundance		2 mM
30	D2O	[U-99% 2H]		10 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details N 191-263 S235F 13C15N + Nsp3 16-111 191-263

#	Name	Isotope labeling	Type	Concentration
31	SARS-CoV-2 Nucleoprotein 191-263 S235F	[U-99% 13C; U-99% 15N]		500 uM
32	SARS-CoV-2 Nsp3 16-111	natural abundance		550 uM
33	sodium phosphate	natural abundance		50 mM
34	sodium chloride	natural abundance		250 mM
35	DTT	natural abundance		2 mM
36	D2O	[U-99% 2H]		10 %

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.13 K, pH 6.5, Details Nsp3 16-111 13C15N + N 191-263

#	Name	Isotope labeling	Type	Concentration
37	SARS-CoV-2 Nsp3 16-111	natural abundance		500 uM
38	SARS-CoV-2 Nucleoprotein 191-263	natural abundance		500 uM
39	sodium phosphate	natural abundance		50 mM
40	sodium chloride	natural abundance		250 mM
41	DTT	natural abundance		2 mM
42	D2O	[U-99% 2H]		10 %

Hide sample codition

Release date

2021-08-09

Citation

The intrinsically disordered SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a
Bessa, L.M., Guseva, S., Camacho-Zarco, A.R., Salvi, N., Maurin, D., Perez, L.M., Botova, M., Malki, A., Nanao, M., Jensen, M.R., Ruigrok, R., Blackledge, M.
Sci. Adv. (2022), 8, eabm4034-eabm4034, PubMed 35044811 , DOI 10.1126/sciadv.abm4034 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 1, : 46 entities Detail

Related entities 2. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 2, : 46 entities Detail

Related entities 3. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 3, : 6 entities Detail

Related entities 4. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 4, : 6 entities Detail

Related entities 5. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 5, : 3 entities Detail

Interaction partners 5. SARS-CoV-2 Nucleoprotein and Nsp3 Ubl1 complex, entity 5, : 1 : 1 interactors Detail

Experiments performed 32 experiments Detail