BMRB: 51070

PRC1_DD

Authors

Tan, F.

Assembly

Prc1 N terminal domain(aa:1-65)

Entity

1. Prc1 N terminal domain(aa:1-65) (polymer, Thiol state: all disulfide bound), 67 monomers, 8034.270 × 2 Da Detail

MRRSEVLAEE SIVCLQKALN HLREIWELIG IPEDQRLQRT EVVKKHIKEL LDMMIAEEES LKERLEH

Total weight

16068.54 Da

Max. entity weight

8034.27 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS14:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.6 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (799 of 836)	94.3 % (415 of 440)	98.4 % (314 of 319)	90.9 % (70 of 77)
Backbone	97.0 % (388 of 400)	94.8 % (127 of 134)	98.5 % (197 of 200)	97.0 % (64 of 66)
Sidechain	95.0 % (477 of 502)	94.1 % (288 of 306)	98.9 % (183 of 185)	54.5 % (6 of 11)
Aromatic	75.0 % (18 of 24)	75.0 % (9 of 12)	81.8 % (9 of 11)	0.0 % (0 of 1)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. entity 1

MRRSEVLAEE SIVCLQKALN HLREIWELIG IPEDQRLQRT EVVKKHIKEL LDMMIAEEES LKERLEH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details in 50 mM phosphate, 150mM NaCl, 5mM DTT at pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	PRC1_DD	[U-100% 13C; U-100% 15N]		0.75 mM
2	PRC1_DD	[U-95% 15N]		0.75 mM
3	phosphate	natural abundance		50 mM
4	NaCl	natural abundance		150 mM
5	DTT	natural abundance		5 mM

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Release date

2021-08-26

Citation

Validation of the solution structure of dimerization domain of PRC1
Tan, F., Xu, J.
PLoS One (2022), 17, e0270572-e0270572, PubMed 35930764 , DOI 10.1371/journal.pone.0270572 , Abstract

Related entities 1. Prc1 N terminal domain(aa:1-65), : 1 : 4 : 15 entities Detail

Interaction partners 1. Prc1 N terminal domain(aa:1-65), : 15 interactors Detail

Experiments performed 4 experiments Detail