BMRB: 51105

Ybt Cy1 D391N

Authors

Marincin, K.A., Mishra, S.H., Frueh, D.P.

Assembly

Ybt Cy1 D391N

Entity

1. Ybt Cy1 D391N (polymer, Thiol state: all free), 453 monomers, 51851.11 Da Detail

MPDESSWPNM TESTPFPLTP VQHAYLTGRM PGQTLGGVGC HLYQEFEGHC LTASQLEQAI TTLLQRHPML HIAFRPDGQQ VWLPQPYWNG VTVHDLRHND AESRQAYLDA LRQRLSHRLL RVEIGETFDF QLTLLPDNRH RLHVNIDLLI MDASSFTLFF DELNALLAGE SLPAIDTRYD FRSYLLHQQK INQPLRDDAR AYWLAKASTL PPAPVLPLAC EPATLREVRN TRRRMIVPAT RWHAFSNRAG EYGVTPTMAL ATCFSAVLAR WGGLTRLLLN ITLFDRQPLH PAVGAMLADF TNILLLDTAC DGDTVSNLAR KNQLTFTEDW EHRHWSGVEL LRELKRQQRY PHGAPVVFTS NLGRSLYSSR AESPLGEPEW GISQTPQVWI NHLAFEHHGE VWLQWDSNDA LFPPALVETL FDAYCQLINQ LCDDESAWQK PFADMLEHHH HHH

Formula weight

51851.11 Da

Source organism

Yersinia pestis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.6 %, Completeness: 28.7 %, Completeness (bb): 55.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	28.7 % (1540 of 5364)	14.3 % (394 of 2758)	37.5 % (786 of 2096)	70.6 % (360 of 510)
Backbone	55.8 % (1484 of 2658)	40.4 % (363 of 898)	57.0 % (762 of 1337)	84.9 % (359 of 423)
Sidechain	2.7 % (84 of 3137)	1.3 % (25 of 1860)	5.0 % (59 of 1190)	0.0 % (0 of 87)
Aromatic	1.5 % (8 of 534)	1.5 % (4 of 267)	1.6 % (4 of 254)	0.0 % (0 of 13)
Methyl	5.6 % (29 of 520)	5.4 % (14 of 260)	5.8 % (15 of 260)

1. entity 1

MPDESSWPNM TESTPFPLTP VQHAYLTGRM PGQTLGGVGC HLYQEFEGHC LTASQLEQAI TTLLQRHPML HIAFRPDGQQ VWLPQPYWNG VTVHDLRHND AESRQAYLDA LRQRLSHRLL RVEIGETFDF QLTLLPDNRH RLHVNIDLLI MDASSFTLFF DELNALLAGE SLPAIDTRYD FRSYLLHQQK INQPLRDDAR AYWLAKASTL PPAPVLPLAC EPATLREVRN TRRRMIVPAT RWHAFSNRAG EYGVTPTMAL ATCFSAVLAR WGGLTRLLLN ITLFDRQPLH PAVGAMLADF TNILLLDTAC DGDTVSNLAR KNQLTFTEDW EHRHWSGVEL LRELKRQQRY PHGAPVVFTS NLGRSLYSSR AESPLGEPEW GISQTPQVWI NHLAFEHHGE VWLQWDSNDA LFPPALVETL FDAYCQLINQ LCDDESAWQK PFADMLEHHH HHH

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Sample

Solvent system 95% H2O/5% D2O, Temperature 298 K, pH 7, Details 796 uM Cy1 D391N; Buffer: 20 mM Sodium Phosphate, 10 mM NaCl, 1 mM EDTA, 5 mM DTT, pH 7.0; 95% H2O/5% D2O; 200 uM DSS.

#	Name	Isotope labeling	Type	Concentration
1	Ybt Cy1 D391N	[U-13C; U-15N; U-2H]		796 uM
2	Sodium Phosphate	natural abundance		20 mM
3	NaCl	natural abundance		10 mM
4	EDTA	natural abundance		1 mM
5	DTT	natural abundance		5 mM

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Release date

2021-09-26

Citation

Global protein dynamics as communication sensors in peptide synthetase domains
Mishra, S.H., Kancherla, A.K., Marincin, K.A., Bouvignies, G., Nerli, S., Sgourakis, N., Dowling, D.P., Frueh, D.P.
Sci. Adv. (2022), 8, eabn6549-eabn6549, PubMed 35857508 , DOI 10.1126/sciadv.abn6549 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Ybt Cy1 D391N, : 1 : 34 entities Detail

Experiments performed 2 experiments Detail